Yanagi Y, Hu H L, Seya T, Yoshikura H
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
Arch Virol. 1994;138(1-2):39-53. doi: 10.1007/BF01310037.
Mouse cell lines (L, NIH3T3, and RMA cells) infected with the Edmonston strain of measles virus (MV) did not exhibit cytopathic effects (CPE), consistent with the finding that mice are not susceptible to MV. Northern blot analysis, however, revealed that MV genes were transcribed in infected L and NIH3T3 cells, although expression levels were much lower than those in lytically infected Vero cells. Expression of MV genes was not detected in infected RMA cells. L and NIH3T3 cells were found to synthesize viral proteins and produce infectious virions after infection. These cell lines did not express on the surface the molecule detectable by antibodies directed against human CD46, the recently identified MV receptor. L cell transfectants expressing human CD46 exhibited CPE after MV infection, and produced 50 times more viral transcripts and 20 times more infectious virions than the parental L cells. The lowest titer of MV that induced viral multiplication in L cells as detected by cocultivation with Vero cells was larger than that in CD46+ L cells by two orders of magnitude. Our results indicate that MV can infect some mouse cells in the absence of CD46, yet the presence of CD46 facilitates multiplication and cytopathogenicity of MV in mouse cells.
感染麻疹病毒（MV）埃德蒙斯顿株的小鼠细胞系（L、NIH3T3和RMA细胞）未表现出细胞病变效应（CPE），这与小鼠对MV不敏感的发现一致。然而，Northern印迹分析显示，MV基因在感染的L和NIH3T3细胞中被转录，尽管表达水平远低于在被裂解感染的Vero细胞中的表达水平。在感染的RMA细胞中未检测到MV基因的表达。发现L和NIH3T3细胞在感染后合成病毒蛋白并产生有感染性的病毒粒子。这些细胞系未在表面表达可被针对人类CD46（最近鉴定出的MV受体）的抗体检测到的分子。表达人类CD46的L细胞转染子在MV感染后表现出CPE，并且产生的病毒转录本比亲代L细胞多50倍，有感染性的病毒粒子多20倍。通过与Vero细胞共培养检测到的在L细胞中诱导病毒增殖的MV最低滴度比在CD46 + L细胞中的低两个数量级。我们的结果表明，在没有CD46的情况下MV可以感染一些小鼠细胞，然而CD46的存在促进了MV在小鼠细胞中的增殖和细胞致病性。
