Valls V, Castelluccio C, Fato R, Genova M L, Bovina C, Saez G, Marchetti M, Parenti Castelli G, Lenaz G
Dipartimento di Biochimica Giovanni Moruzzi, Università di Bologna, Italy.
Biochem Mol Biol Int. 1994 Jul;33(4):633-42.
We have investigated the effect of rat liver perfusion with adriamycin on mitochondrial activities. Although the perfusion treatment per se induces some decline of respiratory activities, adriamycin strongly potentiates this effect; moreover the coenzyme Q9 content of the mitochondrial membrane is significantly lowered by the antibiotic. Coaddition of coenzyme Q10 in the perfusate significantly protects the mitochondria, not only from loss of respiratory activities but also of the endogenous CoQ9 content. Exogenous CoQ10 fails to enhance respiratory activities in control rats, not treated with adriamycin, even though CoQ concentration has been proven not to be kinetically saturating in the respiratory chain under physiological conditions. Thus, the beneficial effect of CoQ10 in the perfusate does not appear to be the result of its role in the respiratory chain but is a consequence of its antioxidant action.
我们研究了用阿霉素灌注大鼠肝脏对线粒体活性的影响。尽管灌注处理本身会导致呼吸活性有所下降，但阿霉素会强烈增强这种作用；此外，抗生素会使线粒体膜的辅酶Q9含量显著降低。在灌注液中同时添加辅酶Q10可显著保护线粒体，不仅能防止呼吸活性丧失，还能防止内源性辅酶Q9含量降低。外源性辅酶Q10在未用阿霉素处理的对照大鼠中无法增强呼吸活性，尽管已证明在生理条件下辅酶Q浓度在呼吸链中动力学上未达到饱和。因此，灌注液中辅酶Q10的有益作用似乎不是其在呼吸链中的作用结果，而是其抗氧化作用的结果。
