Liu H, Köhler J, Fink G R
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Science. 1994 Dec 9;266(5191):1723-6. doi: 10.1126/science.7992058.
A Candida albicans gene (CPH1) was cloned that encodes a protein homologous to Saccharomyces cerevisiae Ste12p, a transcription factor that is the target of the pheromone response mitogen-activated protein kinase cascade. CPH1 complements both the mating defect of ste12 haploids and the filamentous growth defect of ste12/ste12 diploids. Candida albicans strains without a functional CPH1 gene (cph1/cph1) show suppressed hyphal formation on solid medium. However, cph1/cph1 strains can still form hyphae in liquid culture and in response to serum. Thus, filamentous growth may be activated in C. albicans by the same signaling kinase cascade that activates Ste12p in S. cerevisiae; however, alternative pathways may exist in C. albicans.
克隆出了白色念珠菌的一个基因（CPH1），该基因编码一种与酿酒酵母Ste12p同源的蛋白质，Ste12p是一种转录因子，是信息素反应丝裂原活化蛋白激酶级联反应的靶点。CPH1既能弥补ste12单倍体的交配缺陷，也能弥补ste12/ste12二倍体的丝状生长缺陷。没有功能性CPH1基因（cph1/cph1）的白色念珠菌菌株在固体培养基上显示出菌丝形成受到抑制。然而，cph1/cph1菌株在液体培养中以及对血清反应时仍能形成菌丝。因此，白色念珠菌中的丝状生长可能由与酿酒酵母中激活Ste12p相同的信号激酶级联反应激活；然而，白色念珠菌中可能存在其他途径。
