Thamavit W, Pairojkul C, Tiwawech D, Shirai T, Ito N
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok.
Teratog Carcinog Mutagen. 1994;14(4):169-74. doi: 10.1002/tcm.1770140403.
Bile duct hyperplasia caused by proline is believed to represent a chemical effect of the liver fluke, Fasciola hepatica, and the resultant cell division might be expected to play a role as a tumor promoter. To investigate the potential promoting effect of proline on bile duct cancer development, Syrian hamsters were therefore divided into 8 treatment groups: dimethylnitrosamine (DMN) + proline intraperitoneally (i.p.); DMN + proline s.c.; DMN + saline i.p.; DMN + saline s.c.; proline i.p.; proline s.c.; saline i.p.; and saline s.c. DMN was injected i.p. at 20 mg/kg to the animals 2 weeks prior to commencement of proline treatment, whereby 1 ml of a 2 M solution was given by i.p. or s.c. injection 3 times a week for 20 weeks. At the end of week 42, assessment of preneoplastic lesion development did not reveal any significant modulating influence of proline on DMN-initiated lesion development nor did it itself cause persistent bile duct hyperplasia.
脯氨酸引起的胆管增生被认为是肝片吸虫（Fasciola hepatica）的一种化学效应，由此产生的细胞分裂可能被认为起到肿瘤促进剂的作用。为了研究脯氨酸对胆管癌发展的潜在促进作用，叙利亚仓鼠因此被分为8个治疗组：腹腔注射（i.p.）二甲基亚硝胺（DMN）+脯氨酸；皮下注射（s.c.）DMN +脯氨酸；腹腔注射DMN +生理盐水；皮下注射DMN +生理盐水；腹腔注射脯氨酸；皮下注射脯氨酸；腹腔注射生理盐水；皮下注射生理盐水。在开始脯氨酸治疗前2周，给动物腹腔注射20 mg/kg的DMN，然后每周3次通过腹腔或皮下注射给予1 ml 2 M的溶液，持续20周。在第42周末，对癌前病变发展的评估未发现脯氨酸对DMN引发的病变发展有任何显著的调节影响，其本身也未引起持续性胆管增生。
