Zheltikova T M, Mokronosova M A, Gervazieva V B
Zh Mikrobiol Epidemiol Immunobiol. 1994 Jul-Aug(4):100-3.
The method for obtaining allergenic extracts from T. putrescentiae bodies has been developed. As nutrient medium for the cultivation of these mites oat flour has been selected: it has no allergenic cross-reactive properties with T. putrescentiae. Some physico-chemical properties and specific activity of allergenic extracts obtained from these mites were studied. Protein content in these extracts has been found to vary from 0.93 to 0.26 mg/ml, depending on extraction conditions. Protein constitutes 1.5-30.0% of the dry substance of the allergen. After lyophilization the allergen becomes grayish-white powder, readily soluble in water and aqueous saline solutions. In addition, allergenic extracts from T. putrescentiae have pronounced capacity for binding specific IgE antibodies in the sera of patients sensitized to storage mites of this species, which has been confirmed by RAST inhibition and ELISA. Lyophilization produces no changes in the properties and activity of T. putrescentiae allergenic extracts.
已开发出从腐败酪螨虫体中获取变应原提取物的方法。作为培养这些螨虫的营养培养基，已选用燕麦粉：它与腐败酪螨没有变应原交叉反应特性。对从这些螨虫中获得的变应原提取物的一些理化性质和比活性进行了研究。发现这些提取物中的蛋白质含量在0.93至0.26毫克/毫升之间变化，具体取决于提取条件。蛋白质占变应原干物质的1.5 - 30.0%。冻干后，变应原变成灰白色粉末，易溶于水和盐水溶液。此外，来自腐败酪螨的变应原提取物在对该物种仓储螨致敏患者的血清中具有明显的结合特异性IgE抗体的能力，这已通过放射性变应原吸附抑制试验（RAST）和酶联免疫吸附测定（ELISA）得到证实。冻干不会改变腐败酪螨变应原提取物的性质和活性。
