丙型肝炎相关慢性肝炎患者中隐匿性“野生型”和“e 抗原阴性变异型”乙型肝炎病毒感染

Inapparent "wild-type" and "e-minus variant" HBV infection in patients with HCV-related chronic hepatitis.

作者信息

Sardo M A, Rodinò G, Brancatelli S, Pernice M, Campo S, Squadrito G, Russo F, Raimondo G

机构信息

Dipartimento di Medicina Interna, Università di Messina, Italy.

出版信息

Liver. 1994 Oct;14(5):241-4.

PMID:7997082

Abstract

We analysed DNA extracted from liver biopsy specimens and serum samples from 42 HCV-RNA-positive/HBsAg-negative subjects with chronic hepatitis. Twenty-eight of them were anti-HBs/anti-HBc-positive (group A), while 14 were negative for all HBV markers (group B). HBV sequences were found in hepatic DNA of 12 cases (11 of group A, one of group B), but in the serum of only two cases of group A. Sequencing analysis of pre-core region of HBV-DNA showed the presence of wild-type HBV in three cases, HBeAg-defective HBV in three cases, and the coexistence of both viral populations in six cases. These results indicate that HBV and HCV infection may coexist in HBsAg-negative chronic hepatitis, particularly in anti-HBs/anti-HBc-positive patients. However, HBV replication appears suppressed in these cases, and this state of latency may involve both wild and HBeAg-defective HBV types.

摘要

我们分析了42例慢性丙型肝炎病毒（HCV）RNA阳性/乙肝表面抗原（HBsAg）阴性患者肝活检标本和血清样本中提取的DNA。其中28例抗-HBs/抗-HBc阳性（A组），14例所有乙肝病毒（HBV）标志物均为阴性（B组）。在12例患者的肝DNA中发现了HBV序列（A组11例，B组1例），但仅在A组的2例患者血清中检测到。对HBV-DNA前核心区的测序分析显示，3例存在野生型HBV，3例存在HBeAg缺陷型HBV，6例两种病毒群体共存。这些结果表明，HBV和HCV感染可能在HBsAg阴性的慢性肝炎中共存，尤其是在抗-HBs/抗-HBc阳性患者中。然而，在这些病例中HBV复制似乎受到抑制，这种潜伏状态可能涉及野生型和HBeAg缺陷型HBV。