Müller K, Ziereis K
Institut für Pharmazie, Universität Regensburg, Federal Republic of Germany.
Planta Med. 1994 Oct;60(5):421-4. doi: 10.1055/s-2006-959523.
The effects of the extract of the bark of Mahonia aquifolium and its main constituents (berberine, berbamine, oxyacanthine) on 5-lipoxygenase, lipid peroxidation in phospholipid liposomes induced by 2,2'-azo-(bis-2-amidinopropane), deoxyribose degradation, and their reactivities against the free radical 2,2-diphenyl-1-picrylhydrazyl have been studied. The extract of M. aquifolium inhibits 5-LO with an IC50 value of 50 microM, whereas no appreciable effects were observed by its constituent alkaloids. Reactivity against DPPH increased in the following order: berberine < M. aquifolium < oxyacanthine, berbamine. Pro-oxidant effects by M. aquifolium or its constituents can be excluded, since deoxyribose degradation was not influenced as determined by the release of malondialdehyde. The most prominent feature of M. aquifolium is its efficacy in inhibition of lipid peroxidation (IC50 = 5 microM) which was not mediated by the alkaloids berberine, berbamine, and oxyacanthine.
研究了阔叶十大功劳树皮提取物及其主要成分（小檗碱、 berbamine、刺檗碱）对5-脂氧合酶、由2,2'-偶氮-（双-2-脒基丙烷）诱导的磷脂脂质体中的脂质过氧化、脱氧核糖降解以及它们对自由基2,2-二苯基-1-苦基肼的反应活性。阔叶十大功劳提取物抑制5-LO的IC50值为50 microM，而其组成生物碱未观察到明显效果。对DPPH的反应活性按以下顺序增加：小檗碱 < 阔叶十大功劳 < 刺檗碱、berbamine。由于通过丙二醛释放测定的脱氧核糖降解未受影响，因此可以排除阔叶十大功劳或其成分的促氧化作用。阔叶十大功劳最显著的特征是其抑制脂质过氧化的功效（IC50 = 5 microM），这不是由生物碱小檗碱、berbamine和刺檗碱介导的。
