Valiev A G, Kamilov A I, Nizamov F Z
Klin Lab Diagn. 1993 Jul-Aug(4):71-2.
The method is based on the fact that patients' red cells with the specific antigens adsorbed on their surface, interacting with the specific antibodies, enter the hemagglutination reaction. 8% red cell suspension with diluted commercial sera hyperimmune to Salmonellae and Yersinia, serovars O3 and O9, are used in the test. The hemagglutination test was found sufficiently specific and sensitive for the detection of even the lowest concentrations of the specific antigen on the surface of red cell membranes in the patients with salmonellosis and yersiniosis.
表面吸附有特定抗原的患者红细胞与特定抗体相互作用,从而引发血凝反应。检测中使用了8%的红细胞悬液,该悬液与针对沙门氏菌和耶尔森氏菌O3和O9血清型的稀释商业超免疫血清混合。结果发现,血凝试验对于检测患有沙门氏菌病和耶尔森氏菌病患者红细胞膜表面即使是最低浓度的特定抗原也具有足够的特异性和敏感性。