Rattan S I, Clark B F
Department of Chemistry, Aarhus University, Denmark.
Biochem Biophys Res Commun. 1994 Jun 15;201(2):665-72. doi: 10.1006/bbrc.1994.1752.
Kinetin (Kn) is a synthetic cytokinin plant growth hormone having some senescence-retarding effects in plants. Its effects on animal cells have been, until now, best considered as growth inhibitory and anti-tumorigenic. However, we have observed that an addition of 40-200 microM Kn in the culture medium of human diploid fibroblasts can both delay the onset and decrease the extent of many of the ageing characteristics that appear during serial passaging of normal cells in culture. Age-related changes that are affected by Kn include morphological alterations, growth rates, cell size, cytoskeletal organisation, macromolecular synthesis and the intensity of autofluorescence due to the oxidative damage product lipofuscin. These anti-ageing effects of Kn are achieved without any increase in the cell culture lifespan in terms of maximum proliferative capacity in vitro.
激动素（Kn）是一种合成的细胞分裂素类植物生长激素，对植物具有一定的延缓衰老作用。迄今为止，其对动物细胞的影响在很大程度上被认为具有生长抑制和抗肿瘤作用。然而，我们观察到，在人二倍体成纤维细胞的培养基中添加40 - 200微摩尔的激动素，既可以延迟正常细胞在体外连续传代培养过程中出现的许多衰老特征的出现时间，又能降低其出现程度。受激动素影响的与年龄相关的变化包括形态改变、生长速率、细胞大小、细胞骨架组织、大分子合成以及由氧化损伤产物脂褐素引起的自发荧光强度。就体外最大增殖能力而言，激动素的这些抗衰老作用并未使细胞培养寿命有任何增加。
