Rattan S I
Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, University of Aarhus, Denmark.
Biochem Mol Biol Int. 1998 Jul;45(4):753-9. doi: 10.1080/15216549800203162.
The effects of repetitive mild heat shock (30 min, 41 degrees C) on growth and various cellular and biochemical characteristics of human skin fibroblasts undergoing ageing in vitro were analysed. Human skin cells not only tolerated more than 30 repeated heat shocks throughout their replicative lifespan, but also maintained several characteristics of young cells until late in life. Whereas the growth rates, population doubling rates, and cumulative population doubling levels achieved in vitro remained unaffected, age-related changes in cellular morphology, cell size, cytoskeletal organisation, autofluorescence and neutral beta-galactosidase activity were significantly slowed down by repeated mild heat shock. These hormesis-like effects of stress-induced defence processes can be useful to elucidate the role of maintenance and repair mechanisms in ageing.
分析了重复性温和热休克（30分钟，41摄氏度）对体外老化的人皮肤成纤维细胞的生长以及各种细胞和生化特性的影响。人皮肤细胞在其复制寿命期间不仅能耐受30多次重复热休克，而且在生命后期仍保持年轻细胞的若干特性。虽然体外实现的生长速率、群体倍增率和累积群体倍增水平未受影响，但重复性温和热休克显著减缓了细胞形态、细胞大小、细胞骨架组织、自发荧光和中性β-半乳糖苷酶活性等与衰老相关的变化。应激诱导的防御过程的这些类 hormesis 效应有助于阐明维持和修复机制在衰老中的作用。
