辅助性T细胞功能的有限稀释分析。II. 单个辅助性T细胞功能研究的一种方法。
Limiting dilution analysis of helper T-cell function. II. An approach to the study of the function of single helper T cells.
作者信息
Waldmann H, Pope H, Lefkovits I
出版信息
Immunology. 1976 Sep;31(3):343-52.
Procedures have been described which are suitable for experiments utilizing a microculture system whereby one can easily study the function of single helper T cells. These are: (a) the elimination of the background anti-hapten response by preliminary anti-theta + guinea-pig complement complement treatment of the 'hapten-primed' population; (b) the removal of any contaminating hapten-specific B cells from the carrier-primed population by passage through nylon wool; (c) irradiation of long-term primed 'nylon wool' enriched helper cells tp prevent cell division; (d) the partition of individual helper cells by limiting dilution.
已经描述了适用于利用微培养系统进行实验的方法，通过该系统可以轻松研究单个辅助性T细胞的功能。这些方法包括：(a) 通过对 “半抗原致敏” 群体进行预先的抗 θ + 豚鼠补体处理来消除背景抗半抗原反应；(b) 通过尼龙毛柱去除载体致敏群体中任何污染的半抗原特异性B细胞；(c) 照射长期致敏的 “尼龙毛” 富集辅助细胞以防止细胞分裂；(d) 通过有限稀释对单个辅助细胞进行分离。
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