Bocci V, Luzzi E, Corradeschi F, Paulesu L, Rossi R, Cardaioli E, Di Simplicio P
Institute of General Physiology, Faculty of Pharmacy, University of Siena, Italy.
J Biol Regul Homeost Agents. 1993 Oct-Dec;7(4):133-8.
We have investigated the effect of various concentrations of ozone on human blood aiming to correlate the production of cytokines with depletion of reduced glutathione and hemolysis. As erythrocytes constitute the bulk of blood cells and represent the main target of ozone they have been taken as a useful marker of its oxidative activity. It appears that a transient exposure (30 sec) of blood of up to 78 micrograms ozone per ml of blood does not depress the production of cytokines even though there is a slight increase of hemolysis and a small decrease of intracellular reduced glutathione. In contrast either a constant (up to 30 sec) exposure to an ozone flux or a high ozone concentration (108 micrograms/ml) markedly decreases reduced glutathione levels and depresses cytokine production.
我们研究了不同浓度臭氧对人体血液的影响，旨在将细胞因子的产生与还原型谷胱甘肽的消耗及溶血作用联系起来。由于红细胞构成了血细胞的主体，并且是臭氧的主要作用靶点，因此它们被视为臭氧氧化活性的有用标志物。似乎每毫升血液中短暂暴露（30秒）高达78微克臭氧，即使溶血略有增加且细胞内还原型谷胱甘肽略有减少，也不会抑制细胞因子的产生。相比之下，持续（长达30秒）暴露于臭氧通量或高臭氧浓度（108微克/毫升）会显著降低还原型谷胱甘肽水平并抑制细胞因子的产生。
