Jiangsu Province Key Laboratory in Infectious Diseases; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China; Department of Infectious Diseases; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China; China-US Vaccine Research Center; The First Affiliated Hospital of Nanjing Medical University; Nanjing, P.R. China.
Jiangsu Haiyuan Protein Biotech Co. Ltd.; Taizhou, P.R. China.
Hum Vaccin Immunother. 2013 Oct;9(10):2157-64. doi: 10.4161/hv.25656. Epub 2013 Jul 12.
Recent studies have demonstrated that DNA immunization is effective in eliciting antigen-specific antibody responses against a wide range of infectious disease targets. The polyclonal antibodies elicited by DNA vaccination exhibit high sensitivity to conformational epitopes and high avidity. However, there have been limited reports in literature on the production of monoclonal antibodies (mAb) by DNA immunization. Here, by using Clostridium difficile (C. diff) toxin A as a model antigen, we demonstrated that DNA immunization was effective in producing a panel of mAb that are protective against toxin A challenge and can also be used as sensitive reagents to detect toxin A from various testing samples. The immunoglobulin (Ig) gene usage for such mAb was also investigated. Further studies should be conducted to fully establish DNA immunization as a unique platform to produce mAb in various hosts.
最近的研究表明，DNA 免疫在引发针对广泛传染病靶标的抗原特异性抗体反应方面非常有效。DNA 疫苗引发的多克隆抗体对构象表位具有高灵敏度和高亲和力。然而，文献中关于 DNA 免疫产生单克隆抗体 (mAb) 的报道有限。在这里，我们使用艰难梭菌 (C. diff) 毒素 A 作为模型抗原，证明 DNA 免疫可有效产生一组 mAb，这些 mAb 可预防毒素 A 攻击，并且还可作为敏感试剂用于检测来自各种检测样本的毒素 A。我们还研究了此类 mAb 的免疫球蛋白 (Ig) 基因使用情况。应进一步开展研究，以充分确立 DNA 免疫作为在各种宿主中产生 mAb 的独特平台。
