作为一种区分密切相关病原菌的快速方法，对编码核糖体RNA的基因大片段进行扩增和限制性内切酶消化。
Amplification and restriction endonuclease digestion of a large fragment of genes coding for rRNA as a rapid method for discrimination of closely related pathogenic bacteria.
作者信息
Ibrahim A, Gerner-Smidt P, Sjöstedt A
机构信息
Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen, Denmark.
出版信息
J Clin Microbiol. 1996 Dec;34(12):2894-6. doi: 10.1128/jcm.34.12.2894-2896.1996.
Abstract
By use of primers specific to conserved regions of the rRNA gene cluster, a discrete amplicon of approximately 5 kb was amplified by PCR from all 21 bacterial genera investigated. Subsequent endonuclease digestion of the PCR product with HaeIII distinguished between the three species of the human pathogen Francisella spp. on the one hand and four clinically relevant genomic groups of Acinetobacter spp. on the other hand. The described technique has the potential as a rapid method for discriminating between closely related species that are of clinical importance.
摘要
通过使用针对rRNA基因簇保守区域的特异性引物，利用聚合酶链式反应（PCR）从所研究的全部21个细菌属中扩增出了一段约5 kb的离散扩增子。随后用HaeIII对PCR产物进行核酸内切酶消化，可区分人类病原体弗朗西斯菌属的三个种，另一方面也能区分不动杆菌属的四个临床相关基因组群。所描述的技术有潜力成为一种快速鉴别具有临床重要性的密切相关菌种的方法。
相似文献
1
Amplification and restriction endonuclease digestion of a large fragment of genes coding for rRNA as a rapid method for discrimination of closely related pathogenic bacteria.作为一种区分密切相关病原菌的快速方法，对编码核糖体RNA的基因大片段进行扩增和限制性内切酶消化。
J Clin Microbiol. 1996 Dec;34(12):2894-6. doi: 10.1128/jcm.34.12.2894-2896.1996.
2
Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR-amplified fragment of the gene coding for 16S rRNA.通过对编码16S rRNA的基因的PCR扩增片段进行限制性片段长度多态性分析快速鉴定弯曲杆菌属菌种。
J Clin Microbiol. 1996 Jan;34(1):62-7. doi: 10.1128/jcm.34.1.62-67.1996.
3
Molecular technique for rapid identification of mycobacteria.快速鉴定分枝杆菌的分子技术。
J Clin Microbiol. 1996 Jan;34(1):98-102. doi: 10.1128/jcm.34.1.98-102.1996.
4
Identification of Acinetobacter isolates in the A. calcoaceticus-A. baumannii complex by restriction analysis of the 16S-23S rRNA intergenic-spacer sequences.通过对16S-23S rRNA基因间隔序列进行限制性分析，鉴定乙酸钙不动杆菌-鲍曼不动杆菌复合群中的不动杆菌分离株。
J Clin Microbiol. 1995 May;33(5):1108-13. doi: 10.1128/jcm.33.5.1108-1113.1995.
5
16S rRNA PCR followed by restriction endonuclease digestion: a rapid approach for genus level identification of important enteric bacterial pathogens.16S rRNA PCR 加限制性内切酶酶切：一种快速鉴定重要肠道细菌病原体属水平的方法。
J Microbiol Methods. 2013 Dec;95(3):353-6. doi: 10.1016/j.mimet.2013.10.001. Epub 2013 Oct 16.
6
Polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from Rochalimaea species for subtyping.基于聚合酶链反应的罗卡利马氏体物种核糖体操纵子片段的限制性片段长度多态性分析用于亚型分型。
J Clin Microbiol. 1993 Jul;31(7):1730-4. doi: 10.1128/jcm.31.7.1730-1734.1993.
7
Discrimination of relapsing fever Borrelia persica and Borrelia microtti by diagnostic species-specific primers and polymerase chain reaction-restriction fragment length polymorphism.应用诊断种特异性引物和聚合酶链反应-限制性片段长度多态性对回归热螺旋体波斯亚种和微小螺旋体的鉴别。
Vector Borne Zoonotic Dis. 2011 Mar;11(3):201-7. doi: 10.1089/vbz.2009.0170. Epub 2010 Jun 29.
8
Amplification and restriction endonuclease digestion of a large fragment of genes coding for rRNA as a rapid method for discrimination of closely related pathogenic bacteria.作为一种快速鉴别密切相关病原菌的方法，对编码核糖体RNA的基因大片段进行扩增和限制性内切酶消化。
J Clin Microbiol. 1997 Jun;35(6):1646-7. doi: 10.1128/jcm.35.6.1646-1647.1997.
9
Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping.通过聚合酶链反应核糖体分型法确定洋葱伯克霍尔德菌的分子流行病学。
J Clin Microbiol. 1992 Aug;30(8):2084-7. doi: 10.1128/jcm.30.8.2084-2087.1992.
10
Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences.通过限制性内切酶分析以及聚合酶链反应扩增的16S rRNA基因序列的直接DNA循环测序从动物中鉴定分枝杆菌。
J Clin Microbiol. 1993 Dec;31(12):3216-22. doi: 10.1128/jcm.31.12.3216-3222.1993.
引用本文的文献
1
Antibiotic-Resistant Escherichia coli and Class 1 Integrons in Humans, Domestic Animals, and Wild Primates in Rural Uganda.抗生素耐药大肠杆菌和 1 类整合子在乌干达农村的人类、家畜和野生灵长类动物中的情况。
Appl Environ Microbiol. 2018 Oct 17;84(21). doi: 10.1128/AEM.01632-18. Print 2018 Nov 1.
2
Rapid Identification and Characterization of Francisella by Molecular Biology and Other Techniques.通过分子生物学及其他技术对弗朗西斯菌进行快速鉴定与特征分析
Open Microbiol J. 2016 Apr 14;10:64-77. doi: 10.2174/1874285801610010064. eCollection 2016.
3
Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. tularensis subsp. tularensis.对土拉弗朗西斯菌菌株进行的全基因组DNA微阵列分析表明，该物种内存在广泛的遗传保守性，但也鉴定出了高毒力的土拉弗朗西斯菌亚种特有的区域。
J Clin Microbiol. 2003 Jul;41(7):2924-31. doi: 10.1128/JCM.41.7.2924-2931.2003.
4
Identification of major Streptococcal species by rrn-amplified ribosomal DNA restriction analysis.通过rrn扩增核糖体DNA限制性分析鉴定主要链球菌种类。
J Clin Microbiol. 2003 Feb;41(2):657-66. doi: 10.1128/JCM.41.2.657-666.2003.
5
Tularemia.兔热病
Clin Microbiol Rev. 2002 Oct;15(4):631-46. doi: 10.1128/CMR.15.4.631-646.2002.
6
Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing.通过脉冲场凝胶电泳、扩增片段长度多态性指纹图谱和16S rRNA基因测序对土拉弗朗西斯菌菌株进行基因分型。
J Clin Microbiol. 2002 Aug;40(8):2964-72. doi: 10.1128/JCM.40.8.2964-2972.2002.
7
Extensive allelic variation among Francisella tularensis strains in a short-sequence tandem repeat region.土拉弗朗西斯菌菌株在短序列串联重复区域存在广泛的等位基因变异。
J Clin Microbiol. 2001 Sep;39(9):3140-6. doi: 10.1128/JCM.39.9.3140-3146.2001.
8
Evaluation of PCR-based methods for discrimination of Francisella species and subspecies and development of a specific PCR that distinguishes the two major subspecies of Francisella tularensis.基于聚合酶链反应（PCR）的方法用于区分弗朗西斯菌属菌种和亚种的评估以及一种区分土拉弗朗西斯菌两个主要亚种的特异性PCR的开发。
J Clin Microbiol. 2000 Nov;38(11):4180-5. doi: 10.1128/JCM.38.11.4180-4185.2000.
9
Recognition of two novel phenons of the genus Acinetobacter among non-glucose-acidifying isolates from human specimens.在从人类标本中分离出的非葡萄糖酸化菌株中识别不动杆菌属的两种新表型。
J Clin Microbiol. 2000 Nov;38(11):3937-41. doi: 10.1128/JCM.38.11.3937-3941.2000.
10
Amplification and restriction endonuclease digestion of a large fragment of genes coding for rRNA as a rapid method for discrimination of closely related pathogenic bacteria.作为一种快速鉴别密切相关病原菌的方法，对编码核糖体RNA的基因大片段进行扩增和限制性内切酶消化。
J Clin Microbiol. 1997 Jun;35(6):1646-7. doi: 10.1128/jcm.35.6.1646-1647.1997.
本文引用的文献
1
Comparative study of strains of B. tularense in the old and new world and their taxonomy.新旧世界土拉热弗朗西斯菌菌株的比较研究及其分类学
J Hyg Epidemiol Microbiol Immunol. 1959;3:138-49.
2
Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms.基于聚合酶链反应扩增核糖体DNA间隔区多态性的细菌快速鉴定
Appl Environ Microbiol. 1993 Apr;59(4):945-52. doi: 10.1128/aem.59.4.945-952.1993.
3
Analysis of 16S ribosomal DNA sequences of Francisella strains and utilization for determination of the phylogeny of the genus and for identification of strains by PCR.弗朗西斯菌属菌株16S核糖体DNA序列分析及其在该属系统发育测定和通过聚合酶链反应鉴定菌株中的应用。
Int J Syst Bacteriol. 1994 Jan;44(1):38-46. doi: 10.1099/00207713-44-1-38.
4
Comparison of the 23S ribosomal RNA genes and the spacer region between the 16S and 23S rRNA genes of the closely related Mycobacterium avium and Mycobacterium paratuberculosis and the fast-growing Mycobacterium phlei.密切相关的鸟分枝杆菌和副结核分枝杆菌以及快速生长的草分枝杆菌的23S核糖体RNA基因和16S与23S rRNA基因之间间隔区的比较。
Microbiology (Reading). 1994 May;140 ( Pt 5):1103-8. doi: 10.1099/13500872-140-5-1103.
5
Heterogeneity among 16S-23S rRNA intergenic spacers of species within the 'Streptococcus milleri group'.“米勒链球菌群”内各物种16S - 23S rRNA基因间隔区的异质性
Microbiology (Reading). 1995 Jun;141 ( Pt 6):1461-1467. doi: 10.1099/13500872-141-6-1461.
6
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.无需培养的单个微生物细胞的系统发育鉴定和原位检测。
Microbiol Rev. 1995 Mar;59(1):143-69. doi: 10.1128/mr.59.1.143-169.1995.
7
Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds.脆弱埃希氏菌：一种与人类伤口相关的肠杆菌科新菌种。
J Clin Microbiol. 1982 Jun;15(6):1133-40. doi: 10.1128/jcm.15.6.1133-1140.1982.
8
Clinical strains of Acinetobacter classified by DNA-DNA hybridization.通过DNA-DNA杂交分类的不动杆菌临床菌株。
APMIS. 1989 Jul;97(7):595-605. doi: 10.1111/j.1699-0463.1989.tb00449.x.
9
Francisella philomiragia comb. nov. (formerly Yersinia philomiragia) and Francisella tularensis biogroup novicida (formerly Francisella novicida) associated with human disease.费氏弗朗西斯菌（新组合）（原称嗜肺耶尔森菌）和土拉弗朗西斯菌新凶手生物群（原称新凶手弗朗西斯菌）与人类疾病相关。
J Clin Microbiol. 1989 Jul;27(7):1601-8. doi: 10.1128/jcm.27.7.1601-1608.1989.
10
Nature of protective immunity to Francisella tularensis.针对土拉弗朗西斯菌的保护性免疫的本质。
Rev Infect Dis. 1989 May-Jun;11(3):440-51.
Zotero 插件