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本文引用的文献

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Comparative study of strains of B. tularense in the old and new world and their taxonomy.新旧世界土拉热弗朗西斯菌菌株的比较研究及其分类学
J Hyg Epidemiol Microbiol Immunol. 1959;3:138-49.
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Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms.基于聚合酶链反应扩增核糖体DNA间隔区多态性的细菌快速鉴定
Appl Environ Microbiol. 1993 Apr;59(4):945-52. doi: 10.1128/aem.59.4.945-952.1993.
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Analysis of 16S ribosomal DNA sequences of Francisella strains and utilization for determination of the phylogeny of the genus and for identification of strains by PCR.弗朗西斯菌属菌株16S核糖体DNA序列分析及其在该属系统发育测定和通过聚合酶链反应鉴定菌株中的应用。
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Comparison of the 23S ribosomal RNA genes and the spacer region between the 16S and 23S rRNA genes of the closely related Mycobacterium avium and Mycobacterium paratuberculosis and the fast-growing Mycobacterium phlei.密切相关的鸟分枝杆菌和副结核分枝杆菌以及快速生长的草分枝杆菌的23S核糖体RNA基因和16S与23S rRNA基因之间间隔区的比较。
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Heterogeneity among 16S-23S rRNA intergenic spacers of species within the 'Streptococcus milleri group'.“米勒链球菌群”内各物种16S - 23S rRNA基因间隔区的异质性
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Phylogenetic identification and in situ detection of individual microbial cells without cultivation.无需培养的单个微生物细胞的系统发育鉴定和原位检测。
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Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds.脆弱埃希氏菌：一种与人类伤口相关的肠杆菌科新菌种。
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Clinical strains of Acinetobacter classified by DNA-DNA hybridization.通过DNA-DNA杂交分类的不动杆菌临床菌株。
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9
Francisella philomiragia comb. nov. (formerly Yersinia philomiragia) and Francisella tularensis biogroup novicida (formerly Francisella novicida) associated with human disease.费氏弗朗西斯菌（新组合）（原称嗜肺耶尔森菌）和土拉弗朗西斯菌新凶手生物群（原称新凶手弗朗西斯菌）与人类疾病相关。
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作为一种区分密切相关病原菌的快速方法，对编码核糖体RNA的基因大片段进行扩增和限制性内切酶消化。

Amplification and restriction endonuclease digestion of a large fragment of genes coding for rRNA as a rapid method for discrimination of closely related pathogenic bacteria.

作者信息

Ibrahim A, Gerner-Smidt P, Sjöstedt A

机构信息

Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen, Denmark.

出版信息

J Clin Microbiol. 1996 Dec;34(12):2894-6. doi: 10.1128/jcm.34.12.2894-2896.1996.

DOI:10.1128/jcm.34.12.2894-2896.1996
PMID:8940418
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229429/
Abstract

By use of primers specific to conserved regions of the rRNA gene cluster, a discrete amplicon of approximately 5 kb was amplified by PCR from all 21 bacterial genera investigated. Subsequent endonuclease digestion of the PCR product with HaeIII distinguished between the three species of the human pathogen Francisella spp. on the one hand and four clinically relevant genomic groups of Acinetobacter spp. on the other hand. The described technique has the potential as a rapid method for discriminating between closely related species that are of clinical importance.

摘要

通过使用针对rRNA基因簇保守区域的特异性引物，利用聚合酶链式反应（PCR）从所研究的全部21个细菌属中扩增出了一段约5 kb的离散扩增子。随后用HaeIII对PCR产物进行核酸内切酶消化，可区分人类病原体弗朗西斯菌属的三个种，另一方面也能区分不动杆菌属的四个临床相关基因组群。所描述的技术有潜力成为一种快速鉴别具有临床重要性的密切相关菌种的方法。