Monger L E, Nagabhushan M, Pretlow T G, Pretlow T P
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Am J Pathol. 1994 Jul;145(1):54-60.
The controversial and subjective nature of the classification of many prostatic lesions and the increasing recognition that lesions that occur in different topographical regions or zones of the prostate may differ in their biological behavior suggest that new approaches are needed to increase our understanding of the biology of prostatic diseases. The precise recognition and identification of prostatic lesions are prerequisites for studies of their biological behavior. We have developed a novel method for the embedding of whole prostates in glycol methacrylate at low temperatures. Enzyme histochemical procedures that can be conducted with this approach have revealed previously unrecognized features of prostatic intraepithelial neoplasia and have demonstrated the presence of prostatic lesions that resemble enzyme-altered foci that have been reported and characterized extensively in other organ systems.
许多前列腺病变分类具有争议性和主观性，而且人们越来越认识到发生在前列腺不同地形区域或区带的病变在生物学行为上可能存在差异，这表明需要新的方法来增进我们对前列腺疾病生物学的理解。准确识别和鉴定前列腺病变是研究其生物学行为的前提条件。我们开发了一种在低温下将整个前列腺包埋于甲基丙烯酸乙二醇酯中的新方法。用这种方法可以进行的酶组织化学程序揭示了前列腺上皮内瘤变以前未被认识的特征，并证明了存在类似于在其他器官系统中已被广泛报道和表征的酶改变灶的前列腺病变。
