磷脂基曲二糖二甘油酯。粪肠球菌（屎肠球菌）ATCC 9790膜脂磷壁酸的共价连接脂质成分。

Phosphatidylkojibiosyl diglyceride. The covalently linked lipid constituent of the membrane lipoteichoic acid from Streptococcus faecalis (faecium) ATCC 9790.

作者信息

Ganfield M C, Pieringer R A

出版信息

J Biol Chem. 1975 Jan 25;250(2):702-9.

Abstract

Water-soluble [14C]glycerol-labeled lipoteichoic acid isolated from the membrane preparations of Streptococcus faecalis (faecium) ATCC 9790 released chloroform-soluble radioactivity upon mild acid hydrolysis. Chromatography of the radioactive, chloroform-extractable material revealed the presence of a very polar lipid (lipid I). Depending upon the conditions of acid hydrolysis, lipid I accounted for as much as 92% of the total 14C-labeled lipids released. More stringent conditions of acid hydrolysis reduced the amount of lipid I and increased the quantity of the other lipids. Lipid I on further mild acid hydrolysis yielded phosphatidylkojibiosyl diglyceride almost quantitively. The other lipids released from the lipoteichoic acid were kojibiosyl diglyceride, monoglucosyl diglyceride, monoglyceride, diglyceride, and phosphatidylmonoglucosyl diglyceride. These lipids are most likely the degradation products of phosphatidylkojibiosyl diglyceride because they are all simpler, structural derivatives of the phosphoglucolipid and they increase in concentration with increasing acid hydrolysis. These and other data demonstrate that the glycerol phosphate polymer of the membrane lipoteichoic acid of S. faecalis (faecium) ATCC 9790 is covalently linked through a phosphodiester bond to phosphatidylkojibiosyl diglyceride.

摘要

从粪肠球菌（屎肠球菌）ATCC 9790膜制剂中分离得到的水溶性[14C]甘油标记脂磷壁酸，经温和酸水解后释放出氯仿可溶放射性物质。对放射性氯仿可提取物进行色谱分析，发现存在一种极性很强的脂质（脂质I）。根据酸水解条件，脂质I占释放的总14C标记脂质的92%。更严格的酸水解条件会减少脂质I的量，增加其他脂质的量。脂质I经进一步温和酸水解后几乎定量生成磷脂酰kojibiosyl甘油二酯。从脂磷壁酸中释放出的其他脂质有kojibiosyl甘油二酯、单葡萄糖基甘油二酯、单甘油酯、甘油二酯和磷脂酰单葡萄糖基甘油二酯。这些脂质很可能是磷脂酰kojibiosyl甘油二酯的降解产物，因为它们都是这种磷酸葡萄糖脂的更简单的结构衍生物，且随着酸水解程度的增加其浓度也会增加。这些及其他数据表明，粪肠球菌（屎肠球菌）ATCC 9790膜脂磷壁酸的甘油磷酸聚合物通过磷酸二酯键与磷脂酰kojibiosyl甘油二酯共价连接。