各种逆转录病毒Gag蛋白之间相互作用的特异性和序列要求。
Specificity and sequence requirements for interactions between various retroviral Gag proteins.
作者信息
Franke E K, Yuan H E, Bossolt K L, Goff S P, Luban J
机构信息
Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
出版信息
J Virol. 1994 Aug;68(8):5300-5. doi: 10.1128/JVI.68.8.5300-5305.1994.
Abstract
We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the Gag polyproteins tested, only those encoded by closely related retroviruses formed heteromultimers. To determine the primary sequence requirements for human immunodeficiency virus type 1 Gag polyprotein multimerization, we studied the effects on multimerization of deletion and linker insertion mutations. Sequences necessary for this process were located between the C-terminal one-third of the capsid domain and the C terminus of the nucleocapsid domain.
摘要
我们之前建立了一种用于逆转录病毒Gag多聚蛋白多聚化的遗传检测方法（J. 卢班、K. B. 阿林、K. L. 博索尔特、T. 胡马兰和S. P. 戈夫，《病毒学杂志》66:5157 - 5160，1992年）。在此，我们使用该检测方法来证明六种不同逆转录病毒编码的Gag多聚蛋白之间的同聚相互作用。在测试的Gag多聚蛋白中，只有密切相关的逆转录病毒编码的那些形成了异源多聚体。为了确定1型人类免疫缺陷病毒Gag多聚蛋白多聚化的一级序列要求，我们研究了缺失和接头插入突变对多聚化的影响。该过程所需的序列位于衣壳结构域的C末端三分之一与核衣壳结构域的C末端之间。
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