Schepky A G, Bensch K W, Schulz-Knappe P, Forssmann W G
Niedersächsisches Institut für Peptid-Forschung GmbH, Hannover, Germany.
Biomed Chromatogr. 1994 Mar-Apr;8(2):90-4. doi: 10.1002/bmc.1130080209.
Human hemofiltrate (HF) was evaluated regarding its content of free amino acids, proteins, and regulatory peptides. Human HF was obtained from patients with end stage renal disease (ESRD). In contrast to plasma it mainly contains low and middle weight molecules < or = 45 kDa. The content of free amino acids, peptides, and proteins in pooled filtrate was determined by amino acid analysis using ortho-phthaldialdehyde/fluorenyl methyl chloroformate (OPA/FMOC) precolumn derivatization. The total amount of peptides and proteins in human HF is 49.4 mg/L (n = 8). The levels of all free amino acids (230 mg/L) and the concentration of some regulatory peptides like insulin, endothelin, gastrin, vasopressin and angiotensin II were similar compared with blood plasma. The amount of peptides and proteins detected in the filtrate was around 0.07% of total plasma proteins, and consisted mainly of smaller proteins and peptides as shown by size exclusion chromatography (SEC). The presence of large proteins in plasma is reduced by a factor of 1500 after filtration. We conclude that human hemofiltrate is a valuable source for the large-scale extraction of regulatory peptides.
对人血液滤过液（HF）的游离氨基酸、蛋白质和调节肽含量进行了评估。人HF取自终末期肾病（ESRD）患者。与血浆相比，它主要含有分子量小于或等于45 kDa的低分子量和中分子量分子。采用邻苯二甲醛/芴甲氧羰酰氯（OPA/FMOC）柱前衍生化氨基酸分析法测定混合滤过液中游离氨基酸、肽和蛋白质的含量。人HF中肽和蛋白质的总量为49.4 mg/L（n = 8）。与血浆相比，所有游离氨基酸的水平（230 mg/L）以及一些调节肽如胰岛素、内皮素、胃泌素、血管加压素和血管紧张素II的浓度相似。滤过液中检测到的肽和蛋白质的量约为血浆总蛋白的0.07%，如尺寸排阻色谱（SEC）所示，主要由较小的蛋白质和肽组成。过滤后，血浆中大分子蛋白质的含量降低了1500倍。我们得出结论，人血液滤过液是大规模提取调节肽的宝贵来源。
