Stathis C G, Febbraio M A, Carey M F, Snow R J
Exercise Metabolism Unit, Victoria University of Technology, Footscray, Australia.
J Appl Physiol (1985). 1994 Apr;76(4):1802-9. doi: 10.1152/jappl.1994.76.4.1802.
To examine the effect of sprint training on human skeletal muscle purine nucleotide metabolism, eight active untrained subjects completed a maximal 30-s sprint bout on a cycle ergometer before and after 7 wk of sprint training. Resting muscle ATP and total adenine nucleotide content were reduced (P < 0.05) by 19 and 18%, respectively, after training. Training resulted in a 52% attenuation (P < 0.05) in the magnitude of ATP depletion after exercise and a similar reduction (P < 0.05) in the accumulation of inosine 5'-monophosphate and ammonia. During recovery, muscle inosine 5'-monophosphate (P < 0.05) and inosine (P < 0.01) content were reduced after training, as was the accumulation of inosine (P < 0.05). Plasma ammonia was higher (P < 0.05) after training early in recovery; in contrast, plasma hypoxanthine concentrations were reduced (P < 0.05) during the latter stages of recovery. The attenuated resting ATP and total adenine nucleotide contents after training probably result from the acute effects of prior training sessions. The reduction in the magnitude of ATP depletion during a 30-s sprint bout after training must reflect an improved balance between ATP hydrolysis and resynthesis. It is unclear which mechanism(s) is responsible for the reduction in the magnitude of ATP degradation after training.
为研究短跑训练对人体骨骼肌嘌呤核苷酸代谢的影响，8名未受过训练的活跃受试者在进行7周短跑训练前后，在自行车测力计上完成了一次持续30秒的最大强度短跑。训练后，静息肌肉中的ATP和总腺嘌呤核苷酸含量分别降低了19%和18%（P<0.05）。训练使运动后ATP消耗的幅度降低了52%（P<0.05），同时5'-单磷酸肌苷和氨的积累也有类似程度的降低（P<0.05）。在恢复过程中，训练后肌肉中的5'-单磷酸肌苷含量（P<0.05）和肌苷含量（P<0.01）降低，肌苷的积累也减少（P<0.05）。训练后恢复早期血浆氨含量较高（P<0.05）；相反，在恢复后期血浆次黄嘌呤浓度降低（P<0.05）。训练后静息ATP和总腺嘌呤核苷酸含量的降低可能是先前训练课程的急性效应所致。训练后30秒短跑过程中ATP消耗幅度的降低必然反映了ATP水解和再合成之间更好的平衡。目前尚不清楚是哪种机制导致训练后ATP降解幅度降低。
