McColl K A, Gould A R
CSIRO, Australian Animal Health Laboratory, Geelong, Victoria.
Aust Vet J. 1994 Apr;71(4):97-101. doi: 10.1111/j.1751-0813.1994.tb03346.x.
Eight sheep vaccinated with 10(6) pfu of attenuated Australian bluetongue virus serotype 23 (BTV 23A) and eight BTV-free sheep were challenged with virulent BTV 23. There was little subsequent variation in the mean clinical score, or in the mean lymphocyte and platelet concentrations in the peripheral blood of the eight vaccinated sheep. There was a marked thrombocytopenia and lymphopenia in the naive sheep as the mean lymphocyte and platelet concentrations fell to a minimum at days 8 and 11 after inoculation, respectively. Similar changes were observed in three other naive sheep inoculated with field isolates of BTV 1,9 or 23. BTV was detected by nested polymerase chain reaction in whole blood of these sheep between days 6 and 28, in mononuclear leukocytes between days 3 and 14, and in platelets between days 6 and 21.
用10(6) 个蚀斑形成单位（pfu）的减毒澳大利亚23型蓝舌病毒（BTV 23A）对8只绵羊进行接种，并用强毒BTV 23对8只无BTV的绵羊进行攻毒。随后，8只接种疫苗的绵羊的平均临床评分、外周血淋巴细胞和血小板平均浓度几乎没有变化。未接种疫苗的绵羊出现明显的血小板减少和淋巴细胞减少，接种后第8天和第11天，淋巴细胞和血小板平均浓度分别降至最低。在用BTV 1、9或23的田间分离株接种的另外3只未接种疫苗的绵羊中也观察到类似变化。在这些绵羊的全血中，于第6至28天通过巢式聚合酶链反应检测到BTV；在单核白细胞中，于第3至14天检测到BTV；在血小板中，于第6至21天检测到BTV。
