Hlavay J, Haemmerli S D, Guilbault G G
Department of Analytical Chemistry, University of Veszprém, Hungary.
Biosens Bioelectron. 1994;9(3):189-95. doi: 10.1016/0956-5663(94)80121-5.
Fibre-optic biosensors were constructed for determination of hypoxanthine and xanthine. Xanthine oxidase and peroxidase were immobilized on different preactivated membranes which were subsequently mounted onto the tip of a fibre-optic bundle. The H2O2 generated by the reaction of hypoxanthine and xanthine oxidase was measured by chemiluminescence (CL) detection using luminol and peroxidase. A linear calibration curve of the sensors in the range of 1-316 microM hypoxanthine and 3.1-316 microM xanthine, respectively, with a detection limit of 0.55 microM hypoxanthine was obtained. Recovery of hypoxanthine ranged between 91 and 102%.
构建了用于测定次黄嘌呤和黄嘌呤的光纤生物传感器。将黄嘌呤氧化酶和过氧化物酶固定在不同的预活化膜上，随后将这些膜安装在光纤束的尖端。通过使用鲁米诺和过氧化物酶的化学发光（CL）检测来测量次黄嘌呤与黄嘌呤氧化酶反应产生的H2O2。分别获得了传感器在1 - 316 microM次黄嘌呤和3.1 - 316 microM黄嘌呤范围内的线性校准曲线，次黄嘌呤的检测限为0.55 microM。次黄嘌呤的回收率在91%至102%之间。
