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Isolation of motile spermatozoa: comparison of Percoll centrifugation, SpermPrep filtration, and swim-up techniques.

作者信息

Ziebe S, Andersen C Y

机构信息

Department of Obstetrics and Gynaecology, University Hospital of Copenhagen, Denmark.

出版信息

J Assist Reprod Genet. 1993 Oct;10(7):485-7. doi: 10.1007/BF01212940.

DOI:10.1007/BF01212940
PMID:8069093
Abstract
摘要

相似文献

1
Isolation of motile spermatozoa: comparison of Percoll centrifugation, SpermPrep filtration, and swim-up techniques.
J Assist Reprod Genet. 1993 Oct;10(7):485-7. doi: 10.1007/BF01212940.
2
Comparison of sperm preparation methods. Wash and concentration, swim-up, migration-gravity sedimentation, 80% Percoll and semen filtration column.精子制备方法的比较。洗涤与浓缩、上游法、迁移-重力沉降法、80% Percoll法和精液过滤柱法。
J Reprod Med. 1995 May;40(5):342-6.
3
Preparation of oligozoospermic and/or asthenozoospermic semen for intrauterine insemination using the SpermPrep semen filtration column.使用精子制备精液过滤柱制备用于宫腔内人工授精的少弱精子症精液
Fertil Steril. 1992 Apr;57(4):866-70.
4
Human spermatozoa selected by Percoll gradient or swim-up are equally capable of binding to the human zona pellucida and undergoing the acrosome reaction.通过Percoll梯度离心法或上游法筛选出的人类精子，在与人类透明带结合并发生顶体反应的能力上并无差异。
Hum Reprod. 1991 Mar;6(3):401-4. doi: 10.1093/oxfordjournals.humrep.a137349.
5
Sperm selection by PD-10 sephadex columns: comparison with SpermPrep filtration and Percoll centrifugation.通过PD-10葡聚糖凝胶柱进行精子筛选：与SpermPrep过滤法和Percoll离心法的比较
Hum Reprod. 1993 May;8(5):732-6. doi: 10.1093/oxfordjournals.humrep.a138130.
6
Simultaneous swim-up/swim-down of sperm in assisted reproduction procedures.辅助生殖程序中精子的同步上游/下游处理
J Assist Reprod Genet. 1993 May;10(4):261-5. doi: 10.1007/BF01204939.
7
A new method for the purification of human motile spermatozoa applying density-gradient centrifugation: polysucrose media compared to Percoll media.一种应用密度梯度离心法纯化人类活动精子的新方法：聚蔗糖介质与Percoll介质的比较
J Assist Reprod Genet. 1997 Nov;14(10):624-8. doi: 10.1023/a:1022545121367.
8
A Mono-Percoll separation technique improves sperm recovery of normal and male factor specimens when compared with the swim-up technique.与上游法相比，单密度梯度离心分离技术可提高正常和男性因素样本的精子回收率。
Hum Reprod. 1995 Dec;10(12):3195-7. doi: 10.1093/oxfordjournals.humrep.a135886.
9
Comparison of Percoll, mini-Percoll and swim-up methods for sperm preparation from abnormal semen samples.用于从异常精液样本中制备精子的Percoll法、微型Percoll法和上游法的比较。
Hum Reprod. 1992 Feb;7(2):261-6. doi: 10.1093/oxfordjournals.humrep.a137628.
10
Comparison of three sperm preparation media.三种精子处理培养基的比较。
Int J Fertil Womens Med. 1999 May-Jun;44(3):163-7.

引用本文的文献

1
Progesterone-activated spermatozoa used for in vitro fertilization.用于体外受精的孕酮激活精子。
J Assist Reprod Genet. 1998 Mar;15(3):159-61. doi: 10.1023/a:1023065006365.

本文引用的文献

1
Separation of sperm and blood cells during isolation of motile human spermatozoa.在活动的人类精子分离过程中精子与血细胞的分离。
J Assist Reprod Genet. 1993 Feb;10(2):175-6. doi: 10.1007/BF01207745.
2
Removal of bacterial contaminants from semen for in vitro fertilization or artificial insemination by the use of buoyant density centrifugation.通过使用浮力密度离心法从精液中去除细菌污染物用于体外受精或人工授精。
Fertil Steril. 1986 Dec;46(6):1128-32. doi: 10.1016/s0015-0282(16)49893-6.
3
Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques.
J Androl. 1988 Nov-Dec;9(6):367-76. doi: 10.1002/j.1939-4640.1988.tb01067.x.
4
Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia.
J Androl. 1989 May-Jun;10(3):214-20. doi: 10.1002/j.1939-4640.1989.tb00091.x.