Hill-Samli M, MacLeod R M
Proc Soc Exp Biol Med. 1975 Jun;149(2):511-4. doi: 10.3181/00379727-149-38839.
The interaction of thyrotropin-releasing hormone (TRH) and ergocryptine or apomorphine in affecting prolactin secretion was examined. Ergocryptine and apomorphine inhibited the release of 3H-prolactin labeled in an in vitro incubation of the rat anterior pituitary and led to a retention within the gland of 3H-prolactin. TRH (1.4 X 10(-8) M) partially blocked the inhibitory effect of 4 x 10(10) M ergocryptine on 3H-prolactin release and totally blocked the effect on radioimmunoassayable prolactin release. TRH also completely blocked the inhibitory effects of 5 x 10(-8) M apomorphine on both 3H-prolactin and radioimmunoassayable prolactin release. Since TRH can also completely block the inhibitory effects of dopamine upon prolactin secretion, these results are consistent with apomorphine and ergocryptine stimulating "dopaminergic" sites and also further support the role of TRH as a prolactin-releasing factor.
研究了促甲状腺激素释放激素（TRH）与麦角隐亭或阿扑吗啡在影响催乳素分泌方面的相互作用。麦角隐亭和阿扑吗啡在大鼠垂体前叶的体外孵育中抑制了3H-催乳素的释放，并导致3H-催乳素在腺体内潴留。TRH（1.4×10⁻⁸M）部分阻断了4×10⁻¹⁰M麦角隐亭对3H-催乳素释放的抑制作用，并完全阻断了对放射免疫法可检测的催乳素释放的作用。TRH还完全阻断了5×10⁻⁸M阿扑吗啡对3H-催乳素和放射免疫法可检测的催乳素释放的抑制作用。由于TRH也能完全阻断多巴胺对催乳素分泌的抑制作用，这些结果与阿扑吗啡和麦角隐亭刺激“多巴胺能”位点一致，并进一步支持了TRH作为催乳素释放因子的作用。
