McNicol P, Paraskevas M, Guijon F
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
J Med Virol. 1994 Jun;43(2):194-200. doi: 10.1002/jmv.1890430218.
The results of repeated human papillomavirus (HPV) DNA testing were compared to changes in cervical pathology and the composition of vaginal microorganisms. A cohort of 19 women with HPV cervical infections in the absence of cervical intraepithelial neoplasia at enrollment was reexamined on average at 7.3-month intervals over a 2-year period. At each follow-up visit, cytological and colposcopic examinations were done and vaginal microorganisms were assessed quantitatively by Gram staining of secretions, and anaerobic and aerobic culture. HPV genotypes 6, 11, 16, and 18 were detected by polymerase chain reaction analysis using DNA isolated from exfoliated cervical cells. The detection of HPV DNA was significantly associated with carriage of Grade II flora (P < 0.001), isolation of Gardnerella vaginalis (P = 0.03), Ureaplasma urealyticum (P = 0.04), Candida albicans (P = 0.01), Bacteroides species (P = 0.01), and overgrowth by anaerobes (P = 0.004). Normal vaginal flora, characterised by the predominance of Lactobacillus species, was significantly associated (P < 0.001) with a negative HPV test. The detection of HPV DNA is associated with the composition of microorganisms present in the vagina at the time of testing.
将人乳头瘤病毒（HPV）DNA重复检测结果与宫颈病理变化及阴道微生物组成进行了比较。对一组19名入组时存在HPV宫颈感染但无宫颈上皮内瘤变的女性，在2年时间里平均每隔7.3个月进行一次复查。每次随访时，进行细胞学和阴道镜检查，并通过分泌物革兰氏染色、厌氧和需氧培养对阴道微生物进行定量评估。使用从脱落的宫颈细胞中分离的DNA，通过聚合酶链反应分析检测HPV 6、11、16和18型。HPV DNA的检测与II级菌群携带（P<0.001）、阴道加德纳菌分离（P = 0.03）、解脲脲原体（P = 0.04）、白色念珠菌（P = 0.01）、拟杆菌属（P = 0.01）以及厌氧菌过度生长（P = 0.004）显著相关。以乳酸杆菌属占优势为特征的正常阴道菌群与HPV检测阴性显著相关（P<0.001）。HPV DNA的检测与检测时阴道内存在的微生物组成有关。
