Modolell M, Schaible U E, Rittig M, Simon M M
Max-Planck-Institut für Immunbiologie, Freiburg, Germany.
Immunol Lett. 1994 May;40(2):139-46. doi: 10.1016/0165-2478(94)90185-6.
Interaction of B. burgdorferi organisms with mouse bone marrow-derived macrophages (BMM phi) leads to phagocytosis of microorganisms, induction of nitric oxide (NO) and superoxide radicals (O2-) by BMM phi and killing of spirochetes. Destruction of spirochetes by BMM phi was quantified by a new method based on the release of radioactivity from spirochetes pre-labelled with [3H]adenine. Uptake of B. burgdorferi by BMM phi, which mainly occurs by coiling phagocytosis, generation of NO and O2- radicals as well as killing of spirochetes were significantly enhanced by pre-opsonization of spirochetes with monoclonal antibodies (mAb) to the outer surface proteins A and B but not with those to the periplasmic flagellin. Addition of inhibitors specific for NO and O2- radical synthesis either separately or together to cultures of BMM phi and spirochetes resulted in only partial reduction of the killing potential of effector cells. The data indicate that NO and O2- radicals are necessary, but not sufficient, for complete elimination of B. burgdorferi by macrophages. Together with previous findings that protection against B. burgdorferi infection is conveyed by humoral immune responses the present data indicate that one of the important functions of specific antibodies is their participation in macrophage-mediated control of spirochetes.
伯氏疏螺旋体与小鼠骨髓来源的巨噬细胞（BMM phi）相互作用会导致微生物被吞噬、BMM phi诱导产生一氧化氮（NO）和超氧自由基（O2-）以及杀死螺旋体。通过一种基于从预先用[3H]腺嘌呤标记的螺旋体释放放射性的新方法对BMM phi对螺旋体的破坏进行了定量。BMM phi对伯氏疏螺旋体的摄取主要通过卷曲吞噬作用发生，用针对外表面蛋白A和B的单克隆抗体（mAb）对螺旋体进行预调理显著增强了NO和O2-自由基的产生以及螺旋体的杀伤，但对周质鞭毛蛋白的抗体则没有这种作用。单独或一起向BMM phi和螺旋体培养物中添加对NO和O2-自由基合成具有特异性的抑制剂，仅部分降低了效应细胞的杀伤潜力。数据表明，NO和O2-自由基对于巨噬细胞完全清除伯氏疏螺旋体是必要的，但不是充分的。结合先前关于体液免疫反应可提供针对伯氏疏螺旋体感染的保护的发现，目前的数据表明特异性抗体的重要功能之一是它们参与巨噬细胞介导的对螺旋体的控制。
