Rizzo A F, Atroshi F, Ahotupa M, Sankari S, Elovaara E
National Veterinary and Food Research Institute, Department of Chemistry, Helsinki, Finland.
Zentralbl Veterinarmed A. 1994 Mar;41(2):81-90. doi: 10.1111/j.1439-0442.1994.tb00070.x.
An experiment was conducted on rats to investigate the capacity of antioxidants to protect against acute toxicity caused by DON or T-2 toxin. Male rats were fed two different feeds. One group received a feed deficient in vitamins C and E and selenium, whereas the other group was fed with a feed enriched in antioxidants. After two weeks, selected groups of rats were administered orally a single dose of DON or T-2 toxin. After the treatment with mycotoxins, all rats were decapitated. The livers were analyzed for TBARS values, hepatic GSH content and for the activities of CyP-450, CAT, SOD and GSH-TR. Increases in lipid peroxides of 21% and 268% were observed in those rats which did not receive the supplement of antioxidants and which were administered DON or T-2 toxin, respectively. There was no significant increases in the TBARS values in the groups receiving DON with selenium and vitamins, but increases of 57% and 79% were recorded in the groups administered T-2 toxin and antioxidants. Furthermore, in the groups fed the deficient feed and administered DON or T-2 toxin, the lipid peroxidation increased by 33% and 307%, respectively. No mortality, and a lower number of intoxicated animals were observed in rats fed a diet supplemented with antioxidants. Significant decreases of GSH, CAT, SOD, CyP-450 and GSH-TR were recorded in treated rats receiving the deficient feed. The results of this study demonstrate that trichothecenes stimulate lipid peroxidation with consequent decrease of GSH content, but that the dietary use of selenium, alpha-tocopherol and ascorbic acid provides protection against acute toxicosis caused by DON or T-2 toxin.
在大鼠身上进行了一项实验，以研究抗氧化剂预防呕吐毒素（DON）或T-2毒素所致急性毒性的能力。给雄性大鼠喂食两种不同的饲料。一组大鼠的饲料缺乏维生素C、维生素E和硒，而另一组大鼠的饲料富含抗氧化剂。两周后，给选定的几组大鼠口服单剂量的DON或T-2毒素。在用霉菌毒素处理后，将所有大鼠断头处死。分析肝脏的丙二醛（TBARS）值、肝脏谷胱甘肽（GSH）含量以及细胞色素P-450（CyP-450）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽转移酶（GSH-TR）的活性。在未补充抗氧化剂且分别给予DON或T-2毒素的大鼠中，脂质过氧化物分别增加了21%和268%。在同时摄入DON和硒及维生素的组中，TBARS值没有显著增加，但在摄入T-2毒素和抗氧化剂的组中，TBARS值分别增加了57%和79%。此外，在喂食缺乏营养饲料并给予DON或T-2毒素的组中，脂质过氧化分别增加了33%和307%。在喂食补充了抗氧化剂饲料的大鼠中，未观察到死亡情况，且中毒动物数量较少。在接受缺乏营养饲料的处理大鼠中，GSH、CAT、SOD、CyP-450和GSH-TR显著降低。本研究结果表明，单端孢霉烯族毒素会刺激脂质过氧化，从而导致GSH含量降低，但饮食中使用硒、α-生育酚和抗坏血酸可预防由DON或T-2毒素引起的急性中毒。
