Rogers J, Kidd K K
Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.
Am J Phys Anthropol. 1993 Apr;90(4):477-86. doi: 10.1002/ajpa.1330900407.
Yellow baboons (Papio hamadryas cynocephalus) from Mikumi National Park, Tanzania were studied for polymorphisms in nuclear DNA. The study population consists of four social groups that inhabit overlapping home ranges and exchange males. As a result, these groups are considered to be members of a single interbreeding population. Human DNA clones were used as probes to screen five loci (AT3, REN, HEXB, VIM, and APOB) for restriction fragment length polymorphisms (RFLP). A total of 14 polymorphisms, at least one at each locus, was detected in a panel of 27 baboons tested using six restriction enzymes for each locus. Eleven of these RFLP systems have average heterozygosity values greater than 0.40. This initial screening demonstrates that human DNA clones can be used to detect significant numbers of informative DNA polymorphisms in single-copy nuclear genes of this species and suggests that the average proportion of nucleotides polymorphic across nuclear loci in this population may be between 1.0% and 1.3%.
对来自坦桑尼亚米库米国家公园的黄狒狒（阿拉伯狒狒东非亚种）的核DNA多态性进行了研究。研究群体由四个社会群体组成，它们栖息在重叠的活动范围内并交换雄性个体。因此，这些群体被认为是一个单一杂交种群的成员。使用人类DNA克隆作为探针，对五个基因座（AT3、REN、HEXB、VIM和APOB）进行限制性片段长度多态性（RFLP）筛选。在使用每个基因座六种限制性酶测试的27只狒狒样本中，总共检测到14种多态性，每个基因座至少一种。这些RFLP系统中有11个的平均杂合度值大于0.40。这项初步筛选表明，人类DNA克隆可用于检测该物种单拷贝核基因中大量的信息性DNA多态性，并表明该种群中跨核基因座多态性核苷酸的平均比例可能在1.0%至1.3%之间。
