Haavik H I
Acta Pathol Microbiol Scand B. 1975 Oct;83(5):519-24. doi: 10.1111/j.1699-0463.1975.tb00133.x.
The growth of the bacitracin producing strain Bacillus licheniformis AL and the bacitracin-negative mutant SB 319 have been compared at different cultural conditions. Concentrations of the metal chelator EDTA which strongly inhibited the growth of the non-producer only slightly inhibited the growth of the bacitracin producer. The inhibitory effect of EDTA upon SB 319 was reversed by the addition of excess manganese(II)ions, cobalt(II)ions, or zinc(II)ions to the culture. The addition of several other ions had no such effect. The addition of bacitracin to the EDTA inhibited mutant also promoted growth. When the non-producer was mutated back to bacitracin production, the inhibitory effect of EDTA was lost. It is suggested that bacitracin may normally promote the uptake of several trace metals during growth of the producer organism.
已在不同培养条件下比较了杆菌肽产生菌地衣芽孢杆菌AL和杆菌肽阴性突变体SB 319的生长情况。金属螯合剂EDTA的浓度强烈抑制非产生菌的生长，却仅轻微抑制杆菌肽产生菌的生长。向培养物中添加过量的锰（II）离子、钴（II）离子或锌（II）离子可逆转EDTA对SB 319的抑制作用。添加其他几种离子则没有这种效果。向EDTA抑制的突变体中添加杆菌肽也能促进生长。当非产生菌回复突变为产生杆菌肽时，EDTA的抑制作用消失。这表明杆菌肽通常可能在产生菌生长过程中促进几种微量金属的摄取。
