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化学消毒剂及消毒程序检测方法的批判性评估(作者译)

[Critical assessment of methods for testing chemical disinfectants and disinfection procedures (author's transl)].

作者信息

Borneff J, Werner H P, Van de Voorde H, Reybrouck G

出版信息

Zentralbl Bakteriol Orig B. 1975 Aug;160(6):590-600.

PMID:1199526
Abstract

Since the earliest days of disinfectant testing two different methods have to be distinguished: one group simulating practical circumstances the other one allowing the determination of germ reduction, disregarding any practical conditions. At present the assessment of disinfectant substances is carried out according to the results of several methods, done in different countries. Usually standardised suspension-tests with no correspondance to-in-use conditions are done. These are for example the determination of the Rideal-Walker and the Chick Martin coefficient and the capacity test by Kelsey and Sykes as well. Results obtained by the use-dilution method (A.O.A.C.) are also just of theoretical value. The British Standard-Test 3286:1960 and the suspension-test of the Committee on Phytopharmacy (Netherlands), to be preferred in order to state germ reductions, do not imitate particulars of practice either. After establishing recommendations for the various disinfectant procedures, the unification of the test methods on international level should be the next aim. Besides evaluation of disinfectant substances (as it is done by suspension-tests) the testing of disinfectant procedures under conditions, copying those of real life ought to be required. The results of such in-use model tests should answer all the questions arising, for instance, what the concentrations or the exposure-time should be. We would plead in favour of testing disinfectant substances and procedures in two steps - preliminary testing and main tests under in-use conditions for each new preparation. The base for all these tests is the selection of a suitable inactivator.

摘要

自最早进行消毒剂测试以来,有两种不同的方法需要区分:一组模拟实际情况,另一组则不考虑任何实际条件,仅用于确定杀菌率。目前,消毒剂物质的评估是根据不同国家采用的几种方法的结果进行的。通常进行的是与实际使用条件无关的标准化悬液试验。例如,里德尔-沃克系数和奇克·马丁系数的测定以及凯尔西和赛克斯的容量试验。使用稀释法(美国官方分析化学师协会)获得的结果也仅具有理论价值。英国标准试验3286:1960以及植物药剂委员会(荷兰)的悬液试验,尽管在确定杀菌率方面更受青睐,但也没有模拟实际情况的细节。在为各种消毒程序制定建议之后,下一步的目标应该是在国际层面统一测试方法。除了评估消毒剂物质(如通过悬液试验进行的评估)之外,还应该要求在模拟实际生活条件下对消毒程序进行测试。这种实际使用模型测试的结果应该能够回答所有出现的问题,例如浓度或暴露时间应该是多少。我们主张对消毒剂物质和程序分两步进行测试——初步测试以及针对每种新制剂在实际使用条件下的主要测试。所有这些测试的基础是选择合适的灭活剂。

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