Glaeser R M, Downing K H
Molecular and Cell Biology Department, University of California, Berkeley 94720.
Ultramicroscopy. 1993 Dec;52(3-4):478-86. doi: 10.1016/0304-3991(93)90064-5.
Electron diffraction data and high-resolution images can now be used to obtain accurate, three-dimensional density maps of biological macromolecules. These density maps can be interpreted by building an atomic-resolution model of the structure into the experimental density. The Cowley-Moodie formalism of dynamical diffraction theory has been used to validate the use of kinematic diffraction theory (strictly, the weak phase object approximation) in producing such 3D density maps. Further improvements in the preparation of very flat (planar) specimens and in the retention of diffraction to a resolution of 0.2 nm or better could result in electron crystallography becoming as important a technique as X-ray crystallography currently is for the field of structural molecular biology.
电子衍射数据和高分辨率图像现在可用于获得生物大分子精确的三维密度图。通过将结构的原子分辨率模型构建到实验密度中，可以对这些密度图进行解释。动力学衍射理论的考利-穆迪形式已被用于验证运动学衍射理论（严格来说，是弱相位物体近似）在生成此类三维密度图中的应用。在制备非常平整（平面）的标本以及将衍射分辨率保持在0.2纳米或更高方面的进一步改进，可能会使电子晶体学成为结构分子生物学领域与当前X射线晶体学同样重要的技术。
