Ripoche J, Link B, Yucel J K, Tokuyasu K, Malhotra V
Department of Biology, University of California at San Diego, La Jolla 92093.
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1878-82. doi: 10.1073/pnas.91.5.1878.
The role of cytoskeletal elements in the cellularization of syncytial Drosophila embryos is becoming evident; however, the distribution and role of organelles such as the Golgi complex, essential for membrane biogenesis, remain unknown. We have cloned a Golgi-membrane-associated polypeptide, beta-COP, from Drosophila. Immunocytochemical studies of syncytial Drosophila embryos with anti-Drosophila beta-COP antibody reveal that Golgi membranes are spatially segregated from the rapidly dividing nuclei. In early embryos, the Golgi membranes are located in the embryonic cortex and nuclei are confined to the core. This distribution of Golgi membranes may serve in preparation of the embryonic cortex for the accommodation of nuclei upon their eventual migration to the cortex and in biogenesis of the excessive plasma membrane needed for cellularization of syncytial embryos.
细胞骨架成分在果蝇合胞体胚胎细胞化过程中的作用正变得越来越明显；然而，对于膜生物合成至关重要的细胞器，如高尔基体的分布和作用仍不清楚。我们从果蝇中克隆了一种与高尔基体膜相关的多肽β-COP。用抗果蝇β-COP抗体对果蝇合胞体胚胎进行免疫细胞化学研究发现，高尔基体膜在空间上与快速分裂的细胞核分离。在早期胚胎中，高尔基体膜位于胚胎皮质，而细胞核局限于核心区域。高尔基体膜的这种分布可能有助于胚胎皮质为细胞核最终迁移到皮质做好准备，并有助于合胞体胚胎细胞化所需的过量质膜的生物合成。
