Electron microscopic localization of carbonic anhydrase (CA) activity in rabbit cornea.

The localization of CA activity in the rabbit cornea was investigated using a modified Hansson's method. The reaction products indicating CA activity was observed in the lateral membrane of endothelial cell, but not in the apical membrane. In cytoplasm, Golgi complex, and mitochondria of the endothelial cells no specific reaction product could be found. In epithelium, stroma, and Descemet's membrane, no reaction product was seen. The specific staining was inhibited in the control group which was treated with 10(-4)M sodium acetazolamide.