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人类免疫缺陷病毒1型包膜糖蛋白gp120的V1/V2区域在原代巨噬细胞感染及可溶性CD4中和中的功能作用

Functional role of the V1/V2 region of human immunodeficiency virus type 1 envelope glycoprotein gp120 in infection of primary macrophages and soluble CD4 neutralization.

作者信息

Koito A, Harrowe G, Levy J A, Cheng-Mayer C

机构信息

Department of Medicine, University of California, San Francisco 94143-0128.

出版信息

J Virol. 1994 Apr;68(4):2253-9. doi: 10.1128/JVI.68.4.2253-2259.1994.

DOI:10.1128/JVI.68.4.2253-2259.1994
PMID:8139010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC236701/
Abstract

We have examined the influence of the V1/V2 region of the human immunodeficiency virus type 1 (HIV-1) gp120 on certain biologic properties of the virus. We observed that on the genomic background of the T-cell-line-tropic strain, HIV-1SF2mc, both the V1 and V2 domains of the macrophage-tropic strain, HIV-1SF162mc, in addition to the required V3 domain, are necessary to attain full macrophage tropism. Furthermore, the V2 domain modulates the sensitivity of HIV-1 to soluble CD4 neutralization. Structural studies of recombinant and mutant envelope glycoproteins suggest that the function of the V1/V2 region is to interact with the V3 domain and confer on the envelope gp120 of HIV-1SF2mc a conformation more similar to that of the macrophage-tropic strain HIV-1SF162mc. The conformation of the envelope gp120 appears to be strain specific and plays an important role in determining HIV-1 tissue tropism and sensitivity to soluble CD4 neutralization.

摘要

我们研究了人类免疫缺陷病毒1型（HIV-1）gp120的V1/V2区域对该病毒某些生物学特性的影响。我们观察到，在T细胞系嗜性毒株HIV-1SF2mc的基因组背景下，除了所需的V3结构域外，巨噬细胞嗜性毒株HIV-1SF162mc的V1和V2结构域对于实现完全的巨噬细胞嗜性也是必需的。此外，V2结构域可调节HIV-1对可溶性CD4中和作用的敏感性。重组和突变包膜糖蛋白的结构研究表明，V1/V2区域的功能是与V3结构域相互作用，并使HIV-1SF2mc的包膜gp120具有与巨噬细胞嗜性毒株HIV-1SF162mc更相似的构象。包膜gp120的构象似乎具有毒株特异性，并且在决定HIV-1组织嗜性和对可溶性CD4中和作用的敏感性方面起着重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfa8/236701/75b699ac3d7c/jvirol00013-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfa8/236701/0354e1ff2316/jvirol00013-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfa8/236701/75b699ac3d7c/jvirol00013-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfa8/236701/0354e1ff2316/jvirol00013-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfa8/236701/75b699ac3d7c/jvirol00013-0230-a.jpg

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