Adcock J J, Garland L G, Moncada S, Salmon J A
Prostaglandins. 1978 Aug;16(2):163-77. doi: 10.1016/0090-6980(78)90019-9.
Fragments of chopped lung from indomethacin treated guinea-pigs had an anti-aggregating effect when added to human platelet rich plasma (PRP), probably due to the production of prostacyclin (PGI2) since the effect was inhibited by 15-hydroperoxy arachidonic acid (15-HPAA, 10 micrograms ml(-1)). Both 15-HPAA (1-20 micrograms ml(-1) min (-1)) and 13-hydroperoxy linoleic acid (13-HPLA, 20 micrograms ml(-1) min(-1)) caused a marked enhancement of the anaphylactic release of histamine, slow-reacting substance of anaphylaxis (SRS-A) and rabbit aorta contracting substance (RCS) from guinea-pig isolated perfused lungs. This enhancement was not reversed by the concomitant infusion of either PGI2 (5 micrograms ml(-1) min (-1)) or 6-oxo-prostaglandin F1alpha (6-oxo-PGF1alpha, 5 micrograms ml(-1) min(-1)). Anaphylactic release of histamine and SRS-A from guinea-pig perfused lungs was not inhibited by PGI2 (10 ng - 10 microgram ml(-1) min(-1)) but was inhibited by PGE2 (5 and 10 micrograms ml(-1) min (-1)). Antiserum raised to 5,6-dihydro prostacyclin (PGI1) in rabbits, which also binds PGI2, had no effect on the release of anaphylactic mediators. The fatty acid hydroperoxides may enhance mediator release either indirectly by augmenting thromboxane production or by a direct effect on sensitized cells. Further experiments to distinguish between these alternatives are described in the accompanying paper (27).
将消炎痛处理过的豚鼠的肺组织碎片加入到富含人类血小板的血浆（PRP）中时，会产生抗聚集作用，这可能是由于生成了前列环素（PGI2），因为该作用可被15 - 氢过氧花生四烯酸（15 - HPAA，10微克/毫升）抑制。15 - HPAA（1 - 20微克/毫升·分钟）和13 - 氢过氧亚油酸（13 - HPLA，20微克/毫升·分钟）均可显著增强豚鼠离体灌注肺组织中组胺、过敏反应慢反应物质（SRS - A）和兔主动脉收缩物质（RCS）的过敏反应释放。同时输注PGI2（5微克/毫升·分钟）或6 - 氧代 - 前列腺素F1α（6 - 氧代 - PGF1α，5微克/毫升·分钟）并不能逆转这种增强作用。PGI2（10纳克 - 10微克/毫升·分钟）对豚鼠灌注肺组织中组胺和SRS - A的过敏反应释放没有抑制作用，但PGE2（5和10微克/毫升·分钟）可抑制该释放。在兔体内产生的针对5,6 - 二氢前列环素（PGI1）的抗血清（该抗血清也能结合PGI2）对过敏介质的释放没有影响。脂肪酸氢过氧化物可能通过增加血栓素的生成间接增强介质释放，或者对致敏细胞产生直接作用。在随附论文（27）中描述了进一步区分这些可能性的实验。
