Bobrysheva I V, Baron E M, Varshaver N B
Genetika. 1992 Aug;28(8):5-12.
The role of the activated oncogene c-Ha-ras-1 from human bladder carcinoma integrated into the pEJ6.6 plasmid in the mutagenic effect of the plasmid was studied in Chinese hamster cells. The frequency of hypoxanthine-phosphoribosyltransferase defective (HPRT-) mutants after treatment with pEJ6.6 containing an active c-Ha-ras-1 exceeded that in control dishes treated with a derivative of pEJ6.6 plasmid with an inactivated oncogene. The inactivation was achieved by introducing a deletion into the coding region of the oncogene. The mutagenic effect was rather weak but statistically significant. Thus, the data obtained show that the mutagenic activity of pEJ6.6 plasmid is determined by its oncogene. The role of mutagenic effects of activated cellular oncogenes in malignant transformation is discussed.
在中华仓鼠细胞中研究了整合到pEJ6.6质粒中的源自人膀胱癌的活化癌基因c-Ha-ras-1在该质粒诱变效应中的作用。用含有活性c-Ha-ras-1的pEJ6.6处理后，次黄嘌呤-磷酸核糖基转移酶缺陷型（HPRT-）突变体的频率超过了用具有失活癌基因的pEJ6.6质粒衍生物处理的对照培养皿中的频率。通过在癌基因编码区引入缺失实现失活。诱变效应相当弱，但具有统计学意义。因此，所获得的数据表明pEJ6.6质粒的诱变活性由其癌基因决定。讨论了活化的细胞癌基因诱变效应在恶性转化中的作用。
