肠杆菌科的多点鉴定：英国微生物技术学会合作研究报告

Multipoint identification of Enterobacteriaceae: report of the British Society for Microbial Technology collaborative study.

作者信息

Winstanley T G, Limb D I, Wheat P F, Nicol C D

机构信息

Department of Microbiology, Royal Hallamshire Hospital, Sheffield.

出版信息

J Clin Pathol. 1993 Jul;46(7):637-41. doi: 10.1136/jcp.46.7.637.

DOI:10.1136/jcp.46.7.637

PMID:8157751

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC501393/

Abstract

AIMS

To evaluate the accuracy and reproducibility of multipoint identification schemes in a multicentre trial.

METHODS

Forty two strains of Enterobacteriaceae were distributed to 22 laboratories for identification by routine multipoint methods. Analysis of results enabled inter- and intralaboratory reproducibility of a variety of tests, and the ability of laboratories to identify individual organisms to be determined.

RESULTS

Interlaboratory reproducibility of most of the biochemical tests was acceptable. The least reproducible tests, both within and between laboratories, were citrate utilisation, production of urease and beta galactosidase, detection of motility, and decarboxylation of lysine and ornithine. Inconsistent results for these tests were often associated with misidentified strains. Most laboratories performed identifications satisfactorily. Most isolates (72.1%) were identified correctly to species level; 9.6% were incorrectly identified, and 6.4% could not be identified at all. The most difficult organisms to identify were Citrobacter freundii, Enterobacter cloacae, Hafnia alvei and Aeromonas hydrophila. Strains of Enterobacter, Serratia sp, and Providencia sp were difficult to speciate. Several laboratories could not identify organisms exhibiting at least one atypical biochemical reaction.

CONCLUSION

This study emphasises the need for quality control of media and reagents for multipoint identification of Gram negative enteric bacilli.

摘要

目的

在一项多中心试验中评估多点鉴定方案的准确性和可重复性。

方法

将42株肠杆菌科细菌分发给22个实验室，采用常规多点方法进行鉴定。对结果的分析可确定各种试验在实验室间和实验室内部的可重复性，以及各实验室鉴定单个生物体的能力。

结果

大多数生化试验的实验室间可重复性是可接受的。在实验室内部和实验室之间，重复性最差的试验是柠檬酸盐利用试验、脲酶和β-半乳糖苷酶的产生试验、动力检测试验以及赖氨酸和鸟氨酸的脱羧试验。这些试验结果不一致往往与菌株鉴定错误有关。大多数实验室的鉴定结果令人满意。大多数分离株（72.1%）被正确鉴定到种水平；9.6%被错误鉴定，6.4%根本无法鉴定。最难鉴定的生物体是弗氏柠檬酸杆菌、阴沟肠杆菌、蜂房哈夫尼亚菌和嗜水气单胞菌。肠杆菌属、沙雷菌属和普罗威登斯菌属的菌株难以进行种的鉴定。几个实验室无法鉴定出表现出至少一种非典型生化反应的生物体。