Sullivan M P, Cerda J J, Robbins F L, Burgin C W, Beatty R J
Department of Medicine, College of Medicine, University of Florida, Gainesville.
Lab Anim Sci. 1993 Dec;43(6):575-8.
The purpose of this study was to compare the effects of a hyperlipidemic diet on three different rodent models to evaluate them on how their responses simulate human serum lipid diseases. Forty hamsters, 40 gerbils, and 20 guinea pigs were given either a chow diet or a modified high-fat, high-cholesterol diet (HF/HC) for 7 days. Food was withheld from half of the animals on each diet for 12 hours prior to collecting the blood for analysis of total cholesterol and triglyceride concentration. In all species, HF/HC feeding resulted in at least a 370% increase in total cholesterol concentration. Withholding food significantly decreased total cholesterol concentration in hamsters and gerbils fed HF/HC but had no effect on animals fed chow diet. Triglyceride concentrations were increased by the HF/HC in the hamster and the gerbil. As with total cholesterol, triglyceride concentration was decreased after food was withheld in these two animal groups. We suggest that the guinea pig is the most appropriate model for studying hypercholesterolemia because of its moderate plasma cholesterol response and normal triglyceride response to a HF/HC. We also suggest that the hamster is a good model for studying hypertriglyceridemia since increased serum triglyceride concentrations can be easily maintained on a HF/HC.
本研究的目的是比较高脂饮食对三种不同啮齿动物模型的影响，以评估它们对人类血清脂质疾病的模拟反应。将40只仓鼠、40只沙鼠和20只豚鼠分为两组，分别给予普通饮食或改良的高脂肪、高胆固醇饮食（HF/HC），持续7天。在采集血液分析总胆固醇和甘油三酯浓度之前，每种饮食组中有一半的动物禁食12小时。在所有物种中，HF/HC喂养导致总胆固醇浓度至少增加370%。禁食显著降低了喂食HF/HC的仓鼠和沙鼠的总胆固醇浓度，但对喂食普通饮食的动物没有影响。HF/HC使仓鼠和沙鼠的甘油三酯浓度升高。与总胆固醇一样，这两组动物禁食后甘油三酯浓度降低。我们认为豚鼠是研究高胆固醇血症最合适的模型，因为其对HF/HC的血浆胆固醇反应适中，甘油三酯反应正常。我们还认为仓鼠是研究高甘油三酯血症的良好模型，因为在HF/HC饮食下血清甘油三酯浓度很容易升高。
