基于离心的血培养技术：发展阶段
Blood culture technique based on centrifugation: developmental phase.
作者信息
Dorn G L, Haynes J R, Burson G G
出版信息
J Clin Microbiol. 1976 Mar;3(3):251-7. doi: 10.1128/jcm.3.3.251-257.1976.
Abstract
A quantitative and flexible blood culture system based on centrifugation of lysed blood over a density layer is described. The effect of relative centrifugal force, centrifugation time, and two different density solutions on the recovery of Staphylococcus aureus, Escherichia coli, pseudomonas aeruginosa, and Candida albicans was determined. To demonstrate the versatility of this technique, the reisolation of 23 representative microorganisms from inoculated normal human blood was determined at a fixed centrifugation speed and time. The potential merits of this technique are discussed and compared with those of conventional blood culture methods.
摘要
描述了一种基于在密度层上对裂解血液进行离心的定量且灵活的血培养系统。测定了相对离心力、离心时间以及两种不同密度溶液对金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌回收率的影响。为了证明该技术的通用性，在固定的离心速度和时间下，测定了从接种的正常人血液中重新分离出23种代表性微生物的情况。讨论了该技术的潜在优点，并与传统血培养方法的优点进行了比较。
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