Sugimura H, Yamamoto H, Watabiki H, Ogawa H, Harada H, Saitoh I, Miyamura T, Inoue M, Tajima K, Kino I
1st Dept. of Pathology, Hamamatsu University School of Medicine, Japan.
Infection. 1995 Sep-Oct;23(5):258-62. doi: 10.1007/BF01716281.
The hepatitis C virus (HCV) genome was sought in the saliva of 76 chronic HCV carriers (mean age nearly 60 years) in a rural Japanese town, who had high serum titers of c-100 and anti-core second generation antibodies. In 27 samples (27 cases, 36%), the HCV-RNA genome was detected by the reverse transcriptase - polymerase chain reaction with either of two sets of primers covering two regions of the HCV genome: the 5'noncoding region and the region encompassing the putative envelope (E1). Transaminase values at the time of sampling were higher in the patients with than in those without detectable HCV RNA in saliva (p = 0.04 for alanine aminotransferase, p = 0.04 for aspartate aminotransferase; Wilcoxon test). The prevalence of the positivity was higher by 5'noncoding primers (14/59 vs. 15/68). Our data show that the severity and duration of hepatic dysfunction influence the detectability of the HCV genome in the saliva. This has been a controversial point among investigators.
在日本一个乡村小镇，对76名慢性丙型肝炎病毒（HCV）携带者（平均年龄近60岁）的唾液进行了HCV基因组检测，这些携带者血清中c - 100和第二代抗核心抗体滴度较高。在27份样本（27例，36%）中，使用覆盖HCV基因组两个区域（5'非编码区和包含假定包膜蛋白E1的区域）的两组引物之一，通过逆转录 - 聚合酶链反应检测到了HCV - RNA基因组。采样时，唾液中可检测到HCV RNA的患者的转氨酶值高于未检测到的患者（丙氨酸转氨酶p = 0.04，天冬氨酸转氨酶p = 0.04；Wilcoxon检验）。使用5'非编码区引物时阳性率更高（14/59 vs. 15/68）。我们的数据表明，肝功能障碍的严重程度和持续时间会影响唾液中HCV基因组的可检测性。这一直是研究人员之间存在争议的问题。
