Lin H H, Hsu H Y, Chang M H, Chen P J, Chen D S
Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei, Taiwan, R.O.C.
J Pediatr Gastroenterol Nutr. 1993 Aug;17(2):207-10. doi: 10.1097/00005176-199308000-00014.
To investigate the amount of hepatitis B virus (HBV) in colostra of hepatitis B e antigen (HBeAg)-positive carrier mothers and the relationship of HBV amount between colostrum and maternal blood, 50 HBeAg-positive carrier mothers were recruited and studied for hepatitis B surface antigen (HBsAg) titer, HBeAg titer, and HBV-DNA concentration in their sera and colostra. According to the presence or absence of seral HBV-DNA determined by dot hybridization, these 50 HBeAg-positive carrier mothers could be divided into two groups: group 1 (n = 28, HBV-DNA < 0.04 ng/ml), and group 2 (n = 22, HBV-DNA > or = 0.04 ng/ml). The colostral HBsAg and HBeAg titers were both significantly higher in group 2 than in group 1. In addition, both colostral HBsAg and HBeAg titers had positive correlation with each corresponding maternal blood. Although the colostral HBV-DNA was undetectable by dot hybridization, all were positive by polymerase chain reaction with Southern blot hybridization. Because HBV-DNA can be detected in all HBeAg-positive carrier mother's milk, it reinforces the necessity of hepatitis B vaccination for the neonates born to these carrier mothers, particularly in countries with a high carrier rate.
为研究乙肝e抗原（HBeAg）阳性携带者母亲初乳中乙肝病毒（HBV）的含量以及初乳与母血中HBV含量的关系，招募了50名HBeAg阳性携带者母亲，对其血清和初乳中的乙肝表面抗原（HBsAg）滴度、HBeAg滴度及HBV-DNA浓度进行研究。根据斑点杂交法检测血清中HBV-DNA的有无，这50名HBeAg阳性携带者母亲可分为两组：第1组（n = 28，HBV-DNA < 0.04 ng/ml）和第2组（n = 22，HBV-DNA≥0.04 ng/ml）。第2组初乳中的HBsAg和HBeAg滴度均显著高于第1组。此外，初乳中的HBsAg和HBeAg滴度与各自对应的母血均呈正相关。虽然斑点杂交法检测不到初乳中的HBV-DNA，但通过聚合酶链反应及Southern印迹杂交法检测均为阳性。由于在所有HBeAg阳性携带者母亲的乳汁中均可检测到HBV-DNA，这进一步强调了对这些携带者母亲所生新生儿进行乙肝疫苗接种的必要性，尤其是在携带者率较高的国家。
