Tur-Kaspa R, Keshet E, Eliakim M, Shouval D
J Med Virol. 1984;14(1):17-26. doi: 10.1002/jmv.1890140104.
Sera from 153 Israeli patients in various stages of hepatitis B virus (HBV) infection with undetectable hepatitis Be antigen (HBeAg) were studied for the presence of HBV DNA in the serum by molecular hybridization. HBV DNA was detected in 10 patients: 3 with acute hepatitis, 4 asymptomatic hepatitis B surface antigen (HBsAg) carriers, 1 with chronic active hepatitis, 1 with cirrhosis, and 1 with mixed cryoglobulinemia. HBV DNA was detected in 7 of 10 HBeAg-positive control samples tested. Hybridization analysis was used for quantitative comparison of HBV DNA levels in serum. HBV DNA levels, found in HBeAg-negative patients sometimes exceeded the levels found in HBeAg-positive patients. Restriction enzyme analysis of serum HBV DNA from four HBeAg-negative samples gave undistinguishable digestion patterns as compared to 3 HBeAg-positive samples. However, heterogeneity in HBV DNA restriction fragments was detected among HBV genomes in sera of HBeAg-positive samples. These data demonstrate that HBV DNA may be present in the serum at various stages of HBV infection, regardless of HBeAg detection. Failure to detect HBeAg in these patients does not necessarily reflect low serum levels of viral particles, or the occurrence of HBV genome variants.
采用分子杂交法对153例处于不同阶段的乙肝病毒（HBV）感染且乙肝e抗原（HBeAg）检测不到的以色列患者血清进行研究，以检测血清中HBV DNA的存在情况。在10例患者中检测到HBV DNA：3例急性肝炎患者、4例无症状乙肝表面抗原（HBsAg）携带者、1例慢性活动性肝炎患者、1例肝硬化患者和1例混合性冷球蛋白血症患者。在检测的10份HBeAg阳性对照样本中，有7份检测到HBV DNA。采用杂交分析对血清中HBV DNA水平进行定量比较。在HBeAg阴性患者中发现的HBV DNA水平有时超过HBeAg阳性患者中的水平。与3份HBeAg阳性样本相比，对4份HBeAg阴性样本的血清HBV DNA进行限制性内切酶分析，得到的消化模式无法区分。然而，在HBeAg阳性样本的血清HBV基因组中检测到HBV DNA限制性片段存在异质性。这些数据表明，无论能否检测到HBeAg，HBV感染的各个阶段血清中都可能存在HBV DNA。这些患者未检测到HBeAg不一定反映病毒颗粒的血清水平较低，也不一定反映HBV基因组变异的发生。
