大肠杆菌hflA基因座编码一种假定的GTP结合蛋白和两种膜蛋白，其中一种含有类似蛋白酶的结构域。
The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain.
作者信息
Noble J A, Innis M A, Koonin E V, Rudd K E, Banuett F, Herskowitz I
机构信息
Cetus Corporation, Emeryville, CA 94608.
出版信息
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10866-70. doi: 10.1073/pnas.90.22.10866.
Abstract
The hflA (high frequency of lysogenization) locus of Escherichia coli governs the lysis-lysogeny decision of bacteriophage lambda by controlling stability of the phage cII protein. hflA contains three genes, hflX, hflK, and hflC, encoding polypeptides of 50, 46, and 37 kDa, respectively. We have determined the nucleotide sequence of 3843 base pairs containing hflA and have found three large open reading frames corresponding to hflX, hflK, and hflC. HflX contains the three sequence motifs typical of GTP-binding proteins and appears to be a member of a distinct family of putative GTPases. HflC and HflK appear to be integral membrane proteins which show some similarity to each other and to a human membrane protein. The C-terminal region of HflC contains a domain resembling the catalytic domain of ClpP, a bacterial ATP-dependent protease. We hypothesize that HflK and HflC constitute a distinct membrane-bound protease whose activity may be modulated by HflX GTPase.
摘要
大肠杆菌的hflA（高频溶原化）位点通过控制噬菌体cII蛋白的稳定性来决定噬菌体λ的裂解-溶原化。hflA包含三个基因，hflX、hflK和hflC，分别编码50 kDa、46 kDa和37 kDa的多肽。我们测定了包含hflA的3843个碱基对的核苷酸序列，并发现了三个与hflX、hflK和hflC相对应的大的开放阅读框。HflX含有GTP结合蛋白典型的三个序列基序，似乎是一个独特的假定GTP酶家族的成员。HflC和HflK似乎是整合膜蛋白，它们彼此之间以及与一种人类膜蛋白有一些相似性。HflC的C末端区域包含一个类似于ClpP（一种细菌ATP依赖性蛋白酶）催化结构域的结构域。我们推测HflK和HflC构成一种独特的膜结合蛋白酶，其活性可能受HflX GTP酶调节。
相似文献
1
The Escherichia coli hflA locus encodes a putative GTP-binding protein and two membrane proteins, one of which contains a protease-like domain.大肠杆菌hflA基因座编码一种假定的GTP结合蛋白和两种膜蛋白，其中一种含有类似蛋白酶的结构域。
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10866-70. doi: 10.1073/pnas.90.22.10866.
2
Identification of polypeptides encoded by an Escherichia coli locus (hflA) that governs the lysis-lysogeny decision of bacteriophage lambda.鉴定由大肠杆菌基因座（hflA）编码的多肽，该基因座控制噬菌体λ的裂解-溶原决定。
J Bacteriol. 1987 Sep;169(9):4076-85. doi: 10.1128/jb.169.9.4076-4085.1987.
3
Host regulation of lysogenic decision in bacteriophage lambda: transmembrane modulation of FtsH (HflB), the cII degrading protease, by HflKC (HflA).噬菌体λ溶原性决定中的宿主调控：HflKC（HflA）对cII降解蛋白酶FtsH（HflB）的跨膜调节
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5544-9. doi: 10.1073/pnas.94.11.5544.
4
Transcription of the mutL repair, miaA tRNA modification, hfq pleiotropic regulator, and hflA region protease genes of Escherichia coli K-12 from clustered Esigma32-specific promoters during heat shock.在热休克期间，大肠杆菌K-12的mutL修复基因、miaA tRNA修饰基因、hfq多效调节因子基因和hflA区域蛋白酶基因从成簇的西格玛32特异性启动子转录。
J Bacteriol. 1996 Oct;178(19):5719-31. doi: 10.1128/jb.178.19.5719-5731.1996.
5
Properties of HflX, an enigmatic protein from Escherichia coli.来自大肠杆菌的神秘蛋白质HflX的特性
J Bacteriol. 2009 Apr;191(7):2307-14. doi: 10.1128/JB.01353-08. Epub 2009 Jan 30.
6
Cleavage of the cII protein of phage lambda by purified HflA protease: control of the switch between lysis and lysogeny.纯化的HflA蛋白酶对噬菌体λ的cII蛋白的切割：裂解与溶原之间转换的调控
Proc Natl Acad Sci U S A. 1988 Nov;85(21):7882-6. doi: 10.1073/pnas.85.21.7882.
7
Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12.大肠杆菌K-12中hfq插入突变引起的广泛多效性表型的特征分析
Mol Microbiol. 1994 Jul;13(1):35-49. doi: 10.1111/j.1365-2958.1994.tb00400.x.
8
Escherichia coli HflK and HflC can individually inhibit the HflB (FtsH)-mediated proteolysis of lambdaCII in vitro.大肠杆菌 HflK 和 HflC 可分别在体外抑制 HflB（FtsH）介导的 lambdaCII 的蛋白水解。
Arch Biochem Biophys. 2010 Sep 15;501(2):239-43. doi: 10.1016/j.abb.2010.06.030. Epub 2010 Jul 3.
9
Cell growth and lambda phage development controlled by the same essential Escherichia coli gene, ftsH/hflB.细胞生长和λ噬菌体发育受同一个必需的大肠杆菌基因ftsH/hflB控制。
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10861-5. doi: 10.1073/pnas.90.22.10861.
10
hflB, a new Escherichia coli locus regulating lysogeny and the level of bacteriophage lambda cII protein.hflB，一个调控溶原性和噬菌体λ cII蛋白水平的新型大肠杆菌基因座。
J Mol Biol. 1986 Jan 20;187(2):213-24. doi: 10.1016/0022-2836(86)90229-9.
引用本文的文献
1
Characterization of phage vB_EcoS-EE09 infecting DSM613 Isolated from Wastewater Treatment Plant Effluent and Comparative Proteomics of the Infected and Non-Infected Host.感染从污水处理厂流出物中分离出的DSM613的噬菌体vB_EcoS-EE09的特性以及受感染和未受感染宿主的比较蛋白质组学
Microorganisms. 2023 Nov 2;11(11):2688. doi: 10.3390/microorganisms11112688.
2
A novel vibriophage exhibits inhibitory activity against host protein synthesis machinery.一种新型弧菌噬菌体对宿主蛋白合成机制表现出抑制活性。
Sci Rep. 2020 Feb 11;10(1):2347. doi: 10.1038/s41598-020-59396-3.
3
The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.严格反应对 TRAFAC GTPases 和原核核糖体组装的影响。
Cells. 2019 Oct 24;8(11):1313. doi: 10.3390/cells8111313.
4
HflX protein protects from manganese stress.HflX蛋白可抵御锰胁迫。
J Biosci. 2018 Dec;43(5):1001-1013.
5
Structural modules of the stress-induced protein HflX: an outlook on its evolution and biological role.应激诱导蛋白HflX的结构模块：对其进化和生物学作用的展望
Curr Genet. 2019 Apr;65(2):363-370. doi: 10.1007/s00294-018-0905-x. Epub 2018 Nov 17.
6
The universally conserved GTPase HflX is an RNA helicase that restores heat-damaged ribosomes.普遍保守的 GTPase HflX 是一种 RNA 解旋酶，可修复受热损伤的核糖体。
J Cell Biol. 2018 Jul 2;217(7):2519-2529. doi: 10.1083/jcb.201711131. Epub 2018 Jun 21.
7
Characterization of the autophosphorylation property of HflX, a ribosome-binding GTPase from Escherichia coli.来自大肠杆菌的核糖体结合型GTP酶HflX的自身磷酸化特性表征
FEBS Open Bio. 2016 Jun 8;6(7):651-9. doi: 10.1002/2211-5463.12065. eCollection 2016 Jul.
8
The conserved GTPase HflX is a ribosome splitting factor that binds to the E-site of the bacterial ribosome.保守的GTP酶HflX是一种核糖体分裂因子，它与细菌核糖体的E位点结合。
Nucleic Acids Res. 2016 Feb 29;44(4):1952-61. doi: 10.1093/nar/gkv1524. Epub 2016 Jan 4.
9
Differential expression of in vivo and in vitro protein profile of outer membrane of Acidovorax avenae subsp. avenae.活体和体外模式下禾谷寡养单胞菌外膜蛋白图谱的差异表达。
PLoS One. 2012;7(11):e49657. doi: 10.1371/journal.pone.0049657. Epub 2012 Nov 15.
10
The universally conserved prokaryotic GTPases.普遍保守的原核 GTP 酶。
Microbiol Mol Biol Rev. 2011 Sep;75(3):507-42, second and third pages of table of contents. doi: 10.1128/MMBR.00009-11.
本文引用的文献
1
Sequence of the Schizosaccharomyces pombe gtp1 gene and identification of a novel family of putative GTP-binding proteins.粟酒裂殖酵母gtp1基因序列及一类新型假定GTP结合蛋白家族的鉴定。
Gene. 1993 Mar 30;125(2):191-3. doi: 10.1016/0378-1119(93)90327-y.
2
The multicatalytic and 26 S proteases.多催化蛋白酶和26S蛋白酶。
J Biol Chem. 1993 Mar 25;268(9):6065-8.
3
Cell growth and lambda phage development controlled by the same essential Escherichia coli gene, ftsH/hflB.细胞生长和λ噬菌体发育受同一个必需的大肠杆菌基因ftsH/hflB控制。
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10861-5. doi: 10.1073/pnas.90.22.10861.
4
Analysis of membrane and surface protein sequences with the hydrophobic moment plot.利用疏水矩图分析膜蛋白和表面蛋白序列。
J Mol Biol. 1984 Oct 15;179(1):125-42. doi: 10.1016/0022-2836(84)90309-7.
5
The lysis-lysogeny decision of phage lambda: explicit programming and responsiveness.噬菌体λ的裂解-溶原决定：明确的编程与响应性
Annu Rev Genet. 1980;14:399-445. doi: 10.1146/annurev.ge.14.120180.002151.
6
Control of phage lambda development by stability and synthesis of cII protein: role of the viral cIII and host hflA, himA and himD genes.通过cII蛋白的稳定性和合成来控制噬菌体λ的发育：病毒cIII基因以及宿主hflA、himA和himD基因的作用
Cell. 1982 Dec;31(3 Pt 2):565-73. doi: 10.1016/0092-8674(82)90312-9.
7
Factor fraction required for the synthesis of bacteriophage Qbeta-RNA.合成噬菌体Qβ-RNA所需的因子分数。
Nature. 1968 Aug 10;219(5154):588-90. doi: 10.1038/219588a0.
8
Fine structure mapping, complementation, and physiology of Escherichia coli hfl mutants.大肠杆菌hfl突变体的精细结构图谱、互补作用及生理学研究
Genetics. 1974 Jul;77(3):435-48. doi: 10.1093/genetics/77.3.435.
9
The roles of the lambda c3 gene and the Escherichia coli catabolite gene activation system in the establishment of lysogeny by bacteriophage lambda.λ噬菌体c3基因和大肠杆菌分解代谢物基因激活系统在λ噬菌体溶原化建立过程中的作用。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):779-82. doi: 10.1073/pnas.71.3.779.
10
Using PC/GENE for protein and nucleic acid analysis.使用PC/GENE进行蛋白质和核酸分析。
Biotechniques. 1988 Jun;6(6):566-72.
Zotero 插件