采用被动血凝试验对带状疱疹进行诊断及对水痘疫苗进行评估。

Diagnosis of zoster and evaluation of varicella vaccine with a passive haemagglutination assay.

作者信息

Kino Y, Minamishima Y

机构信息

Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan.

出版信息

Vaccine. 1993;11(11):1151-3. doi: 10.1016/0264-410x(93)90078-c.

DOI:10.1016/0264-410x(93)90078-c

PMID:8249435

Abstract

A passive haemagglutination (PHA) assay for the detection of varicella-zoster virus (VZV) antibody was prepared with purified viral glycoproteins. Serum samples from vaccinees with live attenuated varicella vaccine, and of zoster patients, were measured for antibody titres against VZV with PHA, complement fixation (CF) and immune adherence haemagglutination (IAHA) assays, and the results compared. Antibody development could be detected as early as 3 weeks after vaccination, by both PHA and IAHA tests, but not with the CF test. Significant rises in VZV antibody in zoster patients were detected by both PHA and CF tests several days after onset. No cross-reaction was observed using HSV PHA among the vaccinees and the zoster patients. The VZV PHA assay could be used as a monitor of vaccination and a tool for differential diagnosis.

摘要

用纯化的病毒糖蛋白制备了一种用于检测水痘 - 带状疱疹病毒（VZV）抗体的被动血凝（PHA）试验。采用PHA、补体结合（CF）和免疫粘附血凝（IAHA）试验，对接受减毒活水痘疫苗接种者以及带状疱疹患者的血清样本进行VZV抗体滴度检测，并对结果进行比较。PHA和IAHA试验在接种疫苗后3周即可检测到抗体产生，而CF试验则不能。PHA和CF试验在带状疱疹患者发病几天后均检测到VZV抗体显著升高。在接种者和带状疱疹患者中，使用单纯疱疹病毒（HSV）PHA未观察到交叉反应。VZV PHA试验可作为疫苗接种的监测手段和鉴别诊断工具。