Huong V T, Ha D Q, Deubel V
Pasteur Institute, Ho Chi Minh City, Republic of Vietnam.
Am J Trop Med Hyg. 1993 Nov;49(5):538-44. doi: 10.4269/ajtmh.1993.49.538.
Two hundred eighty-five nucleotides from the capsid/premembrane gene region of 16 isolates of Japanese encephalitis (JE) virus from the southern and northern regions of Vietnam obtained during the period 1964-1988 were sequenced and compared with each other and with wild JE virus strains from China and Japan. The 16 Vietnamese isolates showed low rates of genetic evolution (< or = 3.2%) and were classified into one genotype with an overall similarity of > or = 95.4%, including the Japanese JaArOS982 strain taken as reference, and earlier isolated strains from Japan (Nakayama 1935) and China (Beijing-1 1949 and SA14 1954), the wild-type strains used to derive JE vaccines. The implications of the close genetic relationship among Vietnamese strains of JE virus analyzed in our study in relation to their epidemiology and evolution are discussed.
对1964年至1988年期间从越南南部和北部获得的16株日本脑炎（JE）病毒的衣壳/前膜基因区域的285个核苷酸进行了测序，并相互比较，同时与来自中国和日本的野生JE病毒株进行了比较。这16株越南分离株显示出较低的遗传进化率（≤3.2%），并被归为一个基因型，总体相似度≥95.4%，其中包括作为参考的日本JaArOS982株，以及日本（1935年的中山株）和中国（1949年的北京-1株和1954年的SA14株）早期分离的毒株，这些野生型毒株被用于生产JE疫苗。本文讨论了我们研究中分析的越南JE病毒株之间密切的遗传关系对其流行病学和进化的影响。
