Carrillo C, Borca M V, Afonso C L, Onisk D V, Rock D L
Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, New York 11944.
J Virol. 1994 Jan;68(1):580-3. doi: 10.1128/JVI.68.1.580-583.1994.
Long-term persistent infection was established in 100% of pigs (n = 19) experimentally infected with African swine fever virus (ASFV). Viral DNA was detected in peripheral blood mononuclear leukocytes (PBML) at greater than 500 days postinfection by a PCR assay. Infectious virus was not, however, isolated from the same PBML samples. In cell fractionation studies of PBML, monocytes/macrophages were found to harbor viral DNA during the persistent phase of infection. This result indicates that monocytes/macrophages are persistently infected with ASFV and that ASFV-swine monocyte/macrophage interactions can result in either lytic or persistent infection.
100%经非洲猪瘟病毒（ASFV）实验感染的猪（n = 19）都建立了长期持续性感染。通过聚合酶链反应（PCR）检测，在感染后超过500天的外周血单核白细胞（PBML）中检测到病毒DNA。然而，未从相同的PBML样本中分离出传染性病毒。在PBML的细胞分级分离研究中，发现单核细胞/巨噬细胞在感染的持续阶段含有病毒DNA。这一结果表明，单核细胞/巨噬细胞被ASFV持续感染，并且ASFV与猪单核细胞/巨噬细胞的相互作用可导致溶解性感染或持续性感染。
