Taravel M N, Domard A
Laboratoire d'Etudes des Matériaux Plastiques et des Biomatériaux (URA CNRS 507), Université Claude Bernard Lyon I, Villeurbanne, France.
Biomaterials. 1993 Oct;14(12):930-8. doi: 10.1016/0142-9612(93)90135-o.
The interaction between bovine atelocollagen and a high molecular weight fully deacetylated chitosan has been studied. Considering the apparent mass of an anionic equivalent of collagen and the results of various techniques (potentiometry, conductometry and IR spectroscopy), a purely electrostatic mechanism is demonstrated. The role of charge density and chain length of collagen chains is discussed, and although the interaction is weak and hindered by gel formation in collagen solutions, a polyanion-polycation complex is obtained. Circular dichroism spectroscopy experiments show an organization in the solid state of the complex which is quite different to those of collagen and chitosan.
已经研究了牛去端肽胶原蛋白与高分子量完全脱乙酰壳聚糖之间的相互作用。考虑到胶原蛋白阴离子当量的表观质量以及各种技术（电位滴定法、电导法和红外光谱法）的结果，证明了一种纯粹的静电机制。讨论了胶原蛋白链的电荷密度和链长的作用，尽管这种相互作用较弱且受到胶原蛋白溶液中凝胶形成的阻碍，但仍获得了一种聚阴离子-聚阳离子复合物。圆二色光谱实验表明，该复合物在固态下的结构与胶原蛋白和壳聚糖的结构有很大不同。
