Donaldson Amy R, Tanase Constantin Edi, Awuah Dennis, Vasanthi Bathrinarayanan Pranav, Hall Laurence, Nikkhah Mehdi, Khademhosseini Ali, Rose Felicity, Alexander Cameron, Ghaemmaghami Amir M
Immunology and Tissue Modelling Group, School of Life Sciences, University of Nottingham, Nottingham, United Kingdom.
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United States.
Front Bioeng Biotechnol. 2018 Sep 19;6:116. doi: 10.3389/fbioe.2018.00116. eCollection 2018.
Hydrogels are an attractive class of biomaterials in tissue engineering due to their inherently compatible properties for cell culture. Gelatin methacryloyl (GelMA) has shown significant promise in the fields of tissue engineering and drug delivery, as its physical properties can be precisely tuned depending on the specific application. There is a growing appreciation for the interaction between biomaterials and cells of the immune system with the increasing usage of biomaterials for applications. Here, we addressed the current lack of information regarding the immune-modulatory properties of photocrosslinked GelMA. We investigated the ability of human mononuclear cells to mount inflammatory responses in the context of a GelMA hydrogel platform. Using lipopolysaccharide to stimulate a pro-inflammatory immune response, we found tumor necrosis factor-α (TNF-α) expression was suppressed in GelMA culture conditions. Our findings have important implications on the future use of GelMA, and potentially similar hydrogels, and highlight the significance of investigating the potential immune-modulatory properties of biomaterials.
由于水凝胶具有与细胞培养本质上相容的特性，因此在组织工程中是一类有吸引力的生物材料。甲基丙烯酰化明胶（GelMA）在组织工程和药物递送领域已显示出巨大的潜力，因为其物理性质可根据具体应用进行精确调整。随着生物材料在各种应用中的使用日益增加，人们越来越认识到生物材料与免疫系统细胞之间的相互作用。在此，我们解决了目前关于光交联GelMA免疫调节特性信息不足的问题。我们研究了人单核细胞在GelMA水凝胶平台环境中引发炎症反应的能力。使用脂多糖刺激促炎免疫反应，我们发现在GelMA培养条件下肿瘤坏死因子-α（TNF-α）的表达受到抑制。我们的研究结果对GelMA以及潜在的类似水凝胶的未来应用具有重要意义，并突出了研究生物材料潜在免疫调节特性的重要性。
