Soyer H P, Schadendorf D, Cerroni L, Kerl H
Department of Dermatology, University of Graz, Austria.
J Cutan Pathol. 1993 Oct;20(5):411-7. doi: 10.1111/j.1600-0560.1993.tb00663.x.
Verrucous cyst is an uncommon newly described benign epithelial cyst which may be associated with human papillomavirus infection. The purpose of this project was to study the clinicopathological features of verrucous cysts and to demonstrate human papillomavirus (HPV) by polymerase chain reaction (PCR) in these lesions. We have analyzed 5 examples of verrucous cysts: the age range of the patients was between 37-82 years and the most common locations were face and extremities. Clinically the lesions were interpreted as cyst (2), dermatofibroma (2) and basal-cell carcinoma (1). Histopathologic examination of the biopsy specimens showed in all cases an intradermal infundibular cyst lined by a papillated and/or digitated epithelium with focal prominent hypergranulosis and irregular keratohyalin granules. Furthermore, numerous whorls of keratinocytes (so-called squamous eddies) were present within the squamous areas of the hyperplastic epithelium of the cyst, morphologically reminiscent of inverted follicular keratosis. Architectural and cytological features of malignancy were not present. Sections of paraffin-embedded tissue of the 5 verrucous cysts and of 5 "conventional" follicular cysts, infundibular type, were investigated for the presence of HPV DNA sequences by PCR using consensus primers. In all 5 cases of verrucous cysts, HPV-specific DNA sequences were detected, whereas all follicular cysts, infundibular type, were negative. These results support the concept that verrucous cysts can be added to the large spectrum of HPV-induced cutaneous neoplasms. Further studies are needed to identify the specified HPV-type in these distinctive benign cystic proliferations.
疣状囊肿是一种新描述的罕见良性上皮囊肿，可能与人乳头瘤病毒感染有关。本项目的目的是研究疣状囊肿的临床病理特征，并通过聚合酶链反应（PCR）在这些病变中检测人乳头瘤病毒（HPV）。我们分析了5例疣状囊肿：患者年龄在37 - 82岁之间，最常见的部位是面部和四肢。临床上，这些病变被诊断为囊肿（2例）、皮肤纤维瘤（2例）和基底细胞癌（1例）。活检标本的组织病理学检查显示，所有病例均为真皮内漏斗状囊肿，内衬乳头状和/或指状上皮，伴有局灶性明显的颗粒层增厚和不规则的透明角质颗粒。此外，在囊肿增生上皮的鳞状区域内可见大量角质形成细胞漩涡（所谓的鳞状漩涡），形态上类似于倒置性毛囊角化病。未发现恶性肿瘤的结构和细胞学特征。使用通用引物通过PCR对5例疣状囊肿和5例“传统”漏斗状滤泡囊肿的石蜡包埋组织切片进行HPV DNA序列检测。在所有5例疣状囊肿中均检测到HPV特异性DNA序列，而所有漏斗状滤泡囊肿均为阴性。这些结果支持了疣状囊肿可被归入HPV诱导的皮肤肿瘤这一广泛范畴的观点。需要进一步研究以确定这些独特的良性囊性增生中特定的HPV类型。
