Torroni A, Chen Y S, Semino O, Santachiara-Beneceretti A S, Scott C R, Lott M T, Winter M, Wallace D C
Department of Genetics and Molecular Medicine, Emory University School of Medicine, Atlanta, Georgia 30322.
Am J Hum Genet. 1994 Feb;54(2):303-18.
mtDNA sequence variation was examined in 60 Native Americans (Mixtecs from the Alta, Mixtecs from the Baja, Valley Zapotecs, and Highland Mixe) from southern Mexico by PCR amplification and high-resolution restriction endonuclease analysis. Four groups of mtDNA haplotypes (haplogroups A, B, C, and D) characterize Amerind populations, but only three (haplogroups A, B, and C) were observed in these Mexican populations. The comparison of their mtDNA variation with that observed in other populations from Mexico and Central America permits a clear distinction among the different Middle American tribes and raises questions about some of their linguistic affiliations. The males of these population samples were also analyzed for Y-chromosome RFLPs with the probes 49a, 49f, and 12f2. This analysis suggests that certain Y-chromosome haplotypes were brought from Asia during the colonization of the Americas, and a differential gene flow was introduced into Native American populations from European males and females.
通过聚合酶链反应（PCR）扩增和高分辨率限制性内切酶分析，对来自墨西哥南部的60名美洲原住民（来自阿尔塔的米斯特克人、来自巴哈的米斯特克人、山谷萨波特克人和高地米克人）的线粒体DNA（mtDNA）序列变异进行了检测。四组mtDNA单倍型（单倍群A、B、C和D）是美洲印第安人群体的特征，但在这些墨西哥人群体中仅观察到三组（单倍群A、B和C）。将他们的mtDNA变异与在墨西哥和中美洲其他人群中观察到的变异进行比较，可以清楚地区分不同的中美洲部落，并引发了关于他们一些语言归属的问题。还使用探针49a、49f和12f2对这些人群样本中的男性进行了Y染色体限制性片段长度多态性（RFLP）分析。该分析表明，在美洲殖民期间，某些Y染色体单倍型从亚洲传入，并且欧洲男性和女性向美洲原住民群体引入了不同的基因流。
