果蝇中的流浪汉增强子捕获诱变揭示了一种不同于P因子的插入特异性。
hobo enhancer trapping mutagenesis in Drosophila reveals an insertion specificity different from P elements.
作者信息
Smith D, Wohlgemuth J, Calvi B R, Franklin I, Gelbart W M
机构信息
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
出版信息
Genetics. 1993 Dec;135(4):1063-76. doi: 10.1093/genetics/135.4.1063.
Abstract
P element enhancer trapping has become an indispensable tool in the analysis of the Drosophila melanogaster genome. However, there is great variation in the mutability of loci by these elements such that some loci are relatively refractory to insertion. We have developed the hobo transposable element for use in enhancer trapping and we describe the results of a hobo enhancer trap screen. In addition, we present evidence that a hobo enhancer trap element has a pattern of insertion into the genome that is different from the distribution of P elements in the available database. Hence, hobo insertion may facilitate access to genes resistant to P element insertion.
摘要
P 因子增强子捕获已成为黑腹果蝇基因组分析中不可或缺的工具。然而，这些元件在不同位点的诱变能力存在很大差异，以至于一些位点相对难以插入。我们开发了用于增强子捕获的hobo转座元件，并描述了hobo增强子捕获筛选的结果。此外，我们提供的证据表明，hobo增强子捕获元件在基因组中的插入模式与现有数据库中P因子的分布不同。因此，hobo插入可能有助于获取对P因子插入有抗性的基因。
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